Ginkgolide-C-BN-52022-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEGinkgolide CCat. No.: HY-N0785CAS No.: 15291-76-6Synonyms: BN-52022; Ginkgolide-C分式: CHO分量: 440.4作靶點(diǎn): AMPK; Sirtuin; MMP作通路: Epigenetics; PI3K/Akt/mTOR; Cell Cycle/DNA Damage;Metabolic Enzyme/Protease儲(chǔ)存式: Powder -20C 3 years4C 2

2、 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 300 mg/mL (681.20 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 2.2707 mL 11.3533 mL 22.7066 mL5 mM 0.4541 mL 2.2707 mL 4.5413 mL10 mM 0.2271 mL 1.1353 mL 2.2707 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，

3、并請(qǐng)注意儲(chǔ)備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Ginkgolide C從銀杏葉中分離到的 酮類物質(zhì)，具有多種物功能，可以減少板聚集，改善阿爾茲海默癥等。IC50 & Target AMPK MMP-9 Sirtuin1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemE體外研究 Ginkgolide C (3-100M) has no significant effect on 3T3-L1 cell viability, but suppresses adipogenesis in3T3-L1 cells follow

4、ling 24h treatment. Ginkgolide C (10-100M) significantly suppresses lipid accumulationcompared with the control group and also significantly promotes glycerol release in 3T3-L1 adipocytes.Ginkgolide C suppresses PPAR- and PPAR- expression and decreases C/EBP, C/EBP, and SREBP-1cexpression in differe

5、ntiated 3T3-L1 adipocytes. In addition, Ginkgolide C (3-100 M) suppress adipogenesis-related protein (FAS, LPL, and aP2) and mRNA expression in a dose-dependent manner in differentiated3T3-L1 adipocytes. Ginkgolide C (3-100 M) also significantly promotes Sirt1 production and increasesphosphorylation

6、 of AMPK and ACC-1 in a concentration-dependent manner 1. Ginkgolide C (1, 10, 50,100, 500 mM) significantly reduces the collagen (10 mg/mL)-stimulated rat platelet aggregation in a dose-dependent manner. Ginkgolide C (50, 100 mM) causes pro-MMP-9 (92-kDa) to form an activated MMP-9(86-kDa) in colla

7、gen-stimulated platelets 2.PROTOCOLCell Assay 1 3T3-L1 cells are treated with various concentrations of ginkgolide C in 96-well plates for 24h. Cell viability isanalyzed by the MTT assay. The culture medium is removed, and the cells are incubated with 100L MTTsolution (5mg/mL) for 4h at 37C. After p

8、lates are washed, isopropanol is added to dissolve formazonecrystals, followed by absorbance detection with a spectrophotometer at 570nm 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Liou CJ, et al. Ginkgolide C Suppresses Adipogenesis

9、in 3T3-L1 Adipocytes via the AMPK Signaling Pathway. Evid Based ComplementAlternat Med. 2015;2015:298635.2. Ginkgolide C, et al. Ginkgolide C inhibits platelet aggregation in cAMP- and cGMP-dependent manner by activating MMP-9. Biol PharmBull. 2007 Dec;30(12):2340-4.McePdfHeightCaution: Product has not been fully validated for medical