Procarbazine-Hydrochloride-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEProcarbazine HydrochlorideCat. No.: HY-13733CAS No.: 366-70-1分式: CHClNO分量: 257.76作靶點(diǎn): DNA Alkylator/Crosslinker作通路: Cell Cycle/DNA Damage儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 42 m

2、g/mL (162.94 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 3.8796 mL 19.3979 mL 38.7958 mL5 mM 0.7759 mL 3.8796 mL 7.7592 mL10 mM 0.3880 mL 1.9398 mL 3.8796 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Procarbazine Hydrochloride

3、種烷化劑 (alkylator)，具有抗腫瘤的作。體外研究 Procarbazine Hydrochloride is an anticancer agent. Procarbazine is not cytotoxic to the LI 210 cells whichlack cytochrome P-450 or monoamine oxidase activity 1.體內(nèi)研究Procarbazine Hydrochloride (50 mg/kg, i.p.) causes micronuclei in hematopoietic cells, but does not increa

4、se1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEthe lacZ mutant frequency (MF) in bone marrow of mice, similar to that in liver, testis, spleen, kidney, andlung. Procarbazine Hydrochloride (50 mg/kg, i.p.) has positive effect on lung, bone marrow, and spleen forcarcinogenesis 2. Procarbazine (450

5、 mg/kg) significantly decreases testicular and epididymal weight anddrastically reduces haploid cells and spermatogenic arrest in hamster 3.PROTOCOLCell Assay 1 Following Procarbazine or metabolite treatment, cells are diluted to 50.000/mL in 25-cm2 culture flasks (10mL). Every 24 h, a 0.5-mL aliquo

6、t is removed, diluted 20-fold in Hematall isotonic diluent, and the cell numberdetermined with a Coulter Model F electronic cell counter. Counts greater than 10,000/0.5 mL are correctedfor coincidence. Cells are diluted in fresh culture media when cell density exceeded 1 106/mL. Cultures aremaintain

7、ed until the aggregate cell number approached 100 106/mL and doubling time has returned to 12h. Cell survival is determined using Equation A, where TD(doubling time for cells of interest) is 12 h 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal

8、Mice 2Administration 2 Male MutaTM Mouse animals (7-8 weeks old) are used after 2 weeks of acclimation. In the first experiment,18 mice are injected intraperitoneally (i.p.) with 50 mg/kg Procarbazine hydrochloride in 10 mL saline/kg andeight mice are injected with 10 mL saline/kg as the vehicle con

9、trol. Six treated mice are killed 7, 14, and 28days after treatment, and four control mice are killed 7 and 28 days after the treatment. Killing is by cervicaldislocation 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) J Mol Med (Berl). 20

10、19 Jun 14. Mol Imaging Biol. 2019 Apr 15.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Erikson JM, et al. Cytotoxicity and DNA damage caused by the azoxy metabolites of procarbazine in L1210 tumor cells. Cancer Res.1989 Jan 1;49(1):127-33.2. Suzuki T, et al. Procarbazine geno

11、toxicity in the MutaMouse; strong clastogenicity and organ-specific induction of lacZ mutations. MutatRes. 1999 Aug 18;444(2):269-81.3. Weissenberg R, et al. Procarbazine effects on spermatogenesis in golden hamster: a flow cytometric evaluation. Arch Androl. 2002 Mar-Apr;48(2):91-100.McePdfHeightCaution: Product has not b