兒茶素作用機(jī)制 - Medchemexpress - MCE中國

1、Product Data SheetCatechinCat. No.: HY-N0898CAS No.: 154-23-4分式: CHO分量: 290.27作靶點(diǎn): COX; Apoptosis; Influenza Virus; Endogenous Metabolite作通路: Immunology/Inflammation; Apoptosis; Anti-infection; MetabolicEnzyme/Protease儲存式: 4C, sealed storage, away from moisture and light* In solvent : -80C, 6 months

2、; -20C, 1 month (sealed storage, away frommoisture and light)溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 100 mg/mL (344.51 mM)* means soluble, but saturation unknown.SolventMass1 mg 5 mg 10 mgConcentration制備儲備液1 mM 3.4451 mL 17.2253 mL 34.4507 mL5 mM 0.6890 mL 3.4451 mL 6.8901 mL10 mM 0.3445 mL 1.7225 mL 3.4451 mL請根據(jù)產(chǎn)品在不同溶劑中的

3、溶解度選擇合適的溶劑配制儲備液；旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存式和期限：-80C, 6 months; -20C, 1 month (sealed storage, away from moisture and light)。-80C儲存時(shí)，請?jiān)?6 個(gè)內(nèi)使，-20C 儲存時(shí)，請?jiān)?1 個(gè)內(nèi)使。體內(nèi)實(shí)驗(yàn)請根據(jù)您的實(shí)驗(yàn)動物和給藥式選擇適當(dāng)?shù)娜芙獍?。以下溶解案都請先按?In Vitro 式配制澄清的儲備液，再依次添加助溶劑：為保證實(shí)驗(yàn)結(jié)果的可靠性，澄 的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使； 以下溶劑前顯的百分 指該溶劑在您配制

4、終溶液中的體積占；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的式助溶1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (8.61 mM); Clear solution此案可獲得 2.5 mg/mL (8.61 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 25.0 mg/mL 的澄 DMSO 儲備液加到 400 L PEG300 中，混合均勻；向上述體系中加50 L Tween-80，混合均勻；然后繼續(xù)加 450 L 理鹽定容 1 mL。2. 請依

5、序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (8.61 mM); Clear solutionPage 1 of 2 www.MedChemE此案可獲得 2.5 mg/mL (8.61 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 25.0 mg/mL 的澄 DMSO 儲備液加到 900 L 20% 的 SBE-CD 理鹽溶液中，混合均勻。3. 請依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (8.61 mM); Clea

6、r solution此案可獲得 2.5 mg/mL (8.61 mM，飽和度未知) 的澄 溶液，此案不適于實(shí)驗(yàn)周 期在半個(gè)以上的實(shí)驗(yàn)。以 1 mL 作液為例，取 100 L 25.0 mg/mL 的澄 DMSO 儲備液加到 900 L 油中，混合均勻。BIOLOGICAL ACTIVITY物活性 Catechin (+)-Catechin) 抑制環(huán)加氧酶-1 (COX-1) , IC50 為 1.4 M。IC & Target COX-1 Human Endogenous Metabolite1.4 M (IC50)(批量添加)體外研究 Catechin (+)-Catechin) exhib

7、its 95% inhibitory activity at 70 g/mL against cyclooxygenase-1 (COX-1) with an IC50of 1.4 M1.A dose-dependent reduction in color is observed after 24 hours of treatment with Catechin, and 54.76% of the cellsare dead at the highest concentration of Catechin tested (160 g/mL) whereas the IC50 of Cate

8、chin is achieved at127.62 g/mL Catechin. A dose- and time-dependent increase in the induction of apoptosis is observed when MCF-7cells are treated with Catechin. When compare to the control cells at 24 hours, 40.7 and 41.16% of the cells treatedwith 150 g/mL and 300 g/mL Catechin, respectively, unde

9、rgo apoptosis. The expression levels of Caspase-3, -8, and-9 and p53 in MCF-7 cells treated with 150 g/mL Catechin for 24 h increase by 5.81, 1.42, 3.29, and 2.68 fold,respectively, as compare to the levels in untreated control cells2.體內(nèi)研究 Animals treated with Catechin (+)-Catechin) at the lowest te

10、sted dose, i.e., 50 mg/kg, p.o. have spent comparativelymore time in exploring the novel object in the choice trial, however, the difference is not statistically significant. Catechin prevents the time-induced episodic memory deficits in a dose-dependent manner, the most effective being200 mg/kg, p.

11、o. Treatment with Catechin prevents the rise in MPO level compare to DOX alone treatment group(21.989.44 and 36.764.39% in the hippocampus and the frontal cortex respectively)3.PROTOCOLCell Assay 2 The Cell viability assay is performed to assess the toxicity of different concentrations of Catechin o

12、n MCF-7 cells.Briefly, MCF-7 cells (2104 cells/well) are plated in 96-well plates and treated with 0 g/mL Catechin and 160 g/mL Catechin for 24 hours. Then, 40 L of the Cell Titer Blue solution is directly added to the wells and incubated at37C for 6 hours. The fluorescence is recorded with a 560 nm

13、/590 nm (excitation/emission) filter set using amicroplate fluorescence reader, and the IC50 is calculated. Quadruplet samples are run for each concentration ofCatechin in three independent experiments2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.An

14、imal Rats3Administration 3 Twelve weeks old, healthy male rats weighing 200 to 230 g are used in this study. Rats are divided into fourexperimental groups (n=9 each) for one vehicle and three groups of Catechin (three doses). The doses of Catechinare prepared at 50, 100, 200 mg/kg in 0.25% w/v sodiu

15、m carboxy methylcellulose (CMC) and administered orallyfor 7 days prior to and during the experimental trials. Episodic memory, the conscious memory of the pastexperiences is evaluated in this study3.Page 2 of 3 www.MedChemEMCE has not independently confirmed the accuracy of these methods. They are

16、for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Autophagy. 2020 Mar 15:1-15. Plant Cell Physiol. 2019 Oct 23. pii: pcz198. Biochem Biophys Res Commun. 2018 Sep 3;503(1):297-303. Int J Insect Sci. 2018 Feb 28;10:1179543318758409.See more customer validations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. Waffo-Tg

17、uo P, et al. Potential cancer-chemopreventive activities of wine stilbenoids and flavans extracted from grape (Vitis vinifera) cell cultures. NutrCancer. 2001;40(2):173-9.2. Alshatwi AA. Catechin hydrate suppresses MCF-7 proliferation through TP53/Caspase-mediated apoptosis. J Exp Clin Cancer Res. 2010 Dec 17;29:167.3. Cheruku SP, et al. Catechin ameliorates doxorubicin-induced neuronal cytotoxicity in in vitro and episodic memory deficit in in vivo in Wistar