中藥血清藥理學(xué)課件(PPT 14頁(yè))

1、CONTENTS01概述02優(yōu)點(diǎn)03L研究方法04應(yīng)用05綜合評(píng)價(jià)第1頁(yè)，共14頁(yè)。Part 1概述第2頁(yè)，共14頁(yè)。 中藥血清藥理學(xué)是指將中藥或中藥復(fù)方經(jīng)口給動(dòng)物灌服,一定時(shí)間后,采集動(dòng)物血液、分離血清，以含藥血清代替中藥粗提物作為藥物源加入離體反應(yīng)系統(tǒng)中,從而研究其藥理作用的一種半體內(nèi)實(shí)驗(yàn)方法。 1987 年由日本學(xué)者田代真一首次提出。 中藥血清藥理學(xué)的出現(xiàn)在很大程度上克服了中藥復(fù)方及粗提物在體外研究時(shí)的不便，具有可控性好，重復(fù)性高，可以減少各種干擾因素的影響，使得中藥復(fù)方藥理作用機(jī)制的深入研究成為可能。第3頁(yè)，共14頁(yè)。Part 2優(yōu)點(diǎn)第4頁(yè)，共14頁(yè)。1 克服了用中藥制劑直接進(jìn)行體外實(shí)

2、驗(yàn)時(shí), 中藥制劑本身的理化性質(zhì)對(duì)實(shí)驗(yàn)的干擾。3能較直觀地反映藥物在機(jī)體內(nèi)的藥效學(xué)作用。2 直接使用含藥血清進(jìn)行試驗(yàn)，間接避免了直接使用中藥進(jìn)行體外實(shí)驗(yàn)可能得到的陰性結(jié)果。第5頁(yè)，共14頁(yè)。Part 3研究方法第6頁(yè)，共14頁(yè)。性別，動(dòng)物種類、是否造模。原則一是研究含藥血清的加入量來(lái)評(píng)價(jià)血清中藥物的真實(shí)有效濃度;原則二是設(shè)立正常血清空白對(duì)照組來(lái)排除血清本身所含干擾性物質(zhì)對(duì)實(shí)驗(yàn)結(jié)果的影響。動(dòng)物的選擇動(dòng)物的給藥量藥效學(xué)試驗(yàn)體系中含藥血清濃度比，即體積比。量效關(guān)系時(shí)效關(guān)系給藥時(shí)間、取血時(shí)間血清的處理和保存滅活與冰凍處理、其他處理含藥血清加入量和設(shè)立對(duì)照的研究第7頁(yè)，共14頁(yè)。Part 4應(yīng)用第8頁(yè)，共

3、14頁(yè)。骨骼系統(tǒng)抗腫瘤作用抗炎作用抗菌作用 抗病毒生殖系統(tǒng)神經(jīng)系統(tǒng)呼吸系統(tǒng)消化系統(tǒng)泌尿系統(tǒng)心血管系統(tǒng)第9頁(yè)，共14頁(yè)。Objective: To investigate the feasibility of serum pharmacology in evaluating the antitumor effect of Chinese medicine (CM) of Fuzheng Guben, the effects of Fuzheng Yiliu Decoction (扶正抑瘤方, FYD), a typical prescription of Fuzheng Guben, on pr

4、oliferation and apoptosis of hepatoma cells in vitro were observed by two methods with serum pharmacology and traditional pharmacology, respectively.Methods: HepG2 cells were treated with FYDcontaining serum or crude FYD extract in vitro. The proliferation rate was determined by methyl thiazolyl tet

5、razolium(MTT) assay. Cell cycle and apoptosis rate was performed by flfl ow cytometry. And the levels of interleukin-2 (IL-2) and tumor necrosis factor (TNF-) in FYD-containing serum were detected by radioimmunoassay第10頁(yè)，共14頁(yè)。Results:FYD-containing serum remarkably inhibited proliferation and induce

6、d apoptosis of hepatoma cells at least by promoting the production of IL-2 and TNF- in vivo. On the contrary, crude FYD extract promoted the proliferation and did not induce cell apoptosis. The results by serum pharmacology were accordant with those of our previous animal and clinical trials which indicates that serum pharmacology is a reasonable and feasible method for the evaluation of the antitumor effect of herbs of Fuzheng Guben.第11頁(yè)，共14頁(yè)。Part 5綜合評(píng)價(jià)第12頁(yè)，共14頁(yè)。 大部分情況下中藥是口服給藥，在經(jīng)胃腸道和肝臟代謝后，中藥可能早已轉(zhuǎn)化成其他代謝物，所以最后進(jìn)入血液循環(huán)的并非完全是原藥成分。 難以把握最恰當(dāng)?shù)牟裳獣r(shí)間。理論上應(yīng)選擇在藥物濃度的最高峰期取血，但由于中藥成分眾多，中藥復(fù)方更是復(fù)雜多變，不同成分給藥后達(dá)