防御素和急性肺損傷ppt課件

1、防御素與急性肺損傷防御素與急性肺損傷Defensins in Acute Lung InjuryDefinition of ALI & ARDS Acute onset PaO2/FiO2 300 for ALI, 200 for ARDS Bilateral parenchymal infilatrates on chest radiograph No clinical evidence of left atrial hypertensionDefinition of SIRS, Sepsis, MODSSepsisBacteremiaFungemiaParasitemiaVirem

2、iaOtherOtherTraumaBurnsSIRSInfectionPancreatitisSIRS CARSMARSDefensins cysteine-rich cationic antimicrobial peptides with three disulfide bridges-defensins (6) and -defensins (30) antimicrobial activity chemotactic activity for monocytes, immature dendritic cells and memory T cells vasculogenesis in

3、 tumors Role of beta-defensin-1 in ALI Model: Pneumonia & CLP in rat Intervention: Recombinant adenovirus carrying the gene encoding -defensin-2 , intratracheal, 48h prior Monitor: Bacteria clear, inflammation (HE & ICAM), survival ratePathological alteration of lungsCLPCLP & beta-defens

4、in-2Detection of ICAM in lungCLP CLP & beta- defensin 2Level of ICAM-1 in Lung tissue Optical density (arbitr a r y units) 0 12 24 36 72 168 0.400.1 0.2 0.3Group CGroup DTime after CLP (hour)* Time after ALI (hours) 10030509080407060Rats with Ad.rBD2Rats with Ad.VSurvival of adenovirus-pretreated rats following CLP24487296120144168 HrsSurvival %Conclusion and Future -defensin-2 had protective role in ALI/ARDS, via both antibacterial effect and modulation of inflammation Time- & Dose- dependent study, genetic association analysis wi