WATERS公司的UPLC-TQD培訓(xùn)資料MS6調(diào)諧頁(yè)面ppt課件

1、 設(shè)定離子模式Ion mode is set 設(shè)定儀器參數(shù)Instrument parameters are set. API 和 碰撞氣Collision gases ）已經(jīng)調(diào)節(jié)完畢. 先導(dǎo)化合物Parent compounds已經(jīng)確定并優(yōu)化 子離子Daughter ions已經(jīng)決定并優(yōu)化 設(shè)置Mass 分辨率Resolution ） 碰撞能量Collision Energies已優(yōu)化 執(zhí)行采集 儀器調(diào)諧頁(yè)面(MS Tune)使用儀器快捷菜單InstrumentShortcut Item）Use the New, Open, Save or Print tools or use the Fi

2、le menu for these operations.OperateStandbyPurge Refill SyringeStart/Stop 顯示質(zhì)量數(shù)(Da)的范圍或者寬度顯示的中心質(zhì)量數(shù)顯示的信號(hào)增益MS Scan等功能的顯示在底部或者頂部的工具欄雙擊鼠標(biāo)，可以增加或者減少增益的設(shè)置點(diǎn)擊 X 一下，可以恢復(fù)前面的范圍和增益設(shè)置，再點(diǎn)擊一下可以回到缺省的設(shè)置。點(diǎn)擊選擇是否顯示Set = Parent/ precursor IonSet是母離子/先導(dǎo)離子16 data points/amu8 data points/amu4 data points/amu100 101 102 103

3、104100 %94 %97 %Best ResolutionSmooth AppearanceCompromisem/zMM+1100-500 amu - Continuum - 8 pts/amu343344345346347348349350351352353354355356357358359360361362363364365366367368369370m/z0100%0100%0100%Test3 9 (0.190)Scan ES- 3.04e6353.22354.23Test2 9 (0.190)Scan ES- 3.21e6353.23354.33Test1 9 (0.196

4、)Scan ES- 3.65e6353.19354.23355.0916 Data Points / DaltonNo Smoothing8 Data Points / DaltonNo Smoothing4 Data Points / DaltonNo SmoothingProstaglandin PGF2a - ESI negativeAcquisition Mode : ContinuumMass range : 100-500 amuScan Speed : 400 amu/sBASELINE LEVEL=00Intensitym/z BASELINE LEVEL=10Intensit

5、yBASELINE LEVEL=50IntensityIon Current XIon Current Z Ion Current YIon Current Y Ion Current X Baseline Level typically set to 00100020000500100015000500100015000200400600 Set to 0 (Ion Counting Threshold Disabled) Set to 2 (2 time the RSD of noise observed without ion beam) Set to 4 (4 times the RSD of noise observed without ion beam) Set to 10 (10 times the RSD of noise observed without ion beam)Higher IntensityLower IntensityBest Adjust