第八章動物實驗的基本方法

1、徒手固定徒手固定固定板固定固定板固定固定架固定固定架固定簡易固定簡易固定Placing a mouse on a cage lid and grasping the loose skin behind the ears by the thumb and forefingerPush the mouse into the restrainerLeave the tail of the mouse outside the cover of the restrainerLet the rat relaxes on the top of the lid.Stretch the body of the

2、rat by pulling up its tail and then cover the rat with a towel by your left handFold the skirts of towel under the rat from all directions Grasp up the left hindlimb of the rat to expose the abdomen點擊圖片，播放視頻固固定定方方法法Sexing mice - The distance between the anal and genital orifices is greater in the ma

3、le (left) compared to the female (right).Sexing Rat- the distance between the anal and genital orifices is greater in the male (left) compared to the female (right)Slight pressure is applied to the external genitalia to expose the vulva of a female bunnySlight pressure is applied to the external gen

4、italia to expose the penis of a male bunnyA female rabbitA male rabbit點擊圖片，播放視頻經經口口給給藥藥點擊圖片，播放視頻注注射射給給藥藥Disinfect of skin on the top of thigh muscleInsert needle perpendicular to the surface of skin and inject the substance inside the syringeDisinfect of injection site by 75% alcohol directly or 75%

5、 alcohol cotton ballPinch the skin up and insert the needle in for injectionKeep the needle tip in skin for a while after injection to prevent leakage of substance from the needle which due to imbalance pressure of emulsion inside the syringe.Ear vein before administering of tranquilizerEar vein aft

6、er administering of tranquilizerInsert needle into the ear vein with needle tip surface face up點擊圖片，播放視頻采采血血方方法法Fix the position of needle by your left thumb and withdraw blood from the ear veinPress a cotton ball on the top of the injection site before pull out the needle.Apply clip on the top of t

7、he cotton ball for 2-3 minutes to stop further bleeding Apply same procedure as collecting blood from ear vein for blood collection from ear arteryRestrain the anesthetized rabbit on the surgical tableRemove the hair around the neck areaMake a 5 cm opening on the middle line of the neckSearch the ca

8、rotid artery with a tissue forceps and a homeostatic forceps.Isolate the carotid artery from the vagus nerve and other connective tissueTie up the distal end of the carotid artery by silk sutureClip the proximal end of the carotid artery with a bulldog clampInsert a 16 G intravenous catheter into the arteryFix the position of the catheter tip by two silk sutu