常見細(xì)胞種類及培養(yǎng)基

第頁序號細(xì)胞系細(xì)胞類型種組織培養(yǎng)基1293成纖維細(xì)胞人胚胎腎MEM，10%滅活馬血清2IMR-90成纖維細(xì)胞人肺MEM，10%胎牛血清和NEAA3W1-38成纖維細(xì)胞人胚胎肺，3月妊娠MEM,10%胎牛血清4A549上皮細(xì)胞人肺癌F-12K，10%胎牛血清5A431上皮細(xì)胞人表皮細(xì)胞癌DMEM,10%胎牛血清6BHL-100上皮細(xì)胞人乳房McCoy’5A,10%胎牛血清7BeWo上皮滋養(yǎng)層人絨毛癌F-12K,15%胎牛血清8Caco-2上皮細(xì)胞人結(jié)腸腺癌MEM,10%胎牛血清和NEAA9Chang上皮細(xì)胞人肝臟BME,10%小牛血清10HCT-15上皮細(xì)胞人結(jié)腸直腸腺癌RPMI-1640,10%胎牛血清11HeLa上皮細(xì)胞人子宮頸癌MEM,10%胎牛血清和NEAA(insuspension,S-MEM)12HEp-G2上皮細(xì)胞人肝細(xì)胞癌MEM,10%胎牛血清和NEAA13HEp-2上皮細(xì)胞人喉癌MEM,10%胎牛血清;或RPMI-1640,10%胎牛血清14HT-1080上皮細(xì)胞人纖維肉瘤MEM,10%HI胎牛血清和NEAA15HT-29上皮細(xì)胞人結(jié)腸腺癌McCoy’5A,10%胎牛血清16JEG-2上皮細(xì)胞人絨毛膜癌MEM,10%胎牛血清17MCF7上皮細(xì)胞人乳腺癌MEM,10%胎牛血清NEAA,10ug/ml胰島素18KB上皮細(xì)胞人口腔癌MEM,10%胎牛血清和NEAA19Saos-2上皮細(xì)胞人骨肉瘤McCoy’5A,15%胎牛血清20WI-38上皮細(xì)胞人胚胎肺BME,10%胎牛血清21WISH上皮細(xì)胞人羊膜BME,10%胎牛血清22WS1人胚胎皮膚MEM,10%胎牛血清和NEAA23HUVEC內(nèi)皮細(xì)胞人臍帶F-12K,10%胎牛血清和肝素鹽100ug/ml24EB-3淋巴樣人Burkitt淋巴瘤RPMI-1640,10%胎牛血清25Raji淋巴樣人Burkitt淋巴瘤RPMI-1640,10%胎牛血清26IM-9成淋巴細(xì)胞人骨髓瘤病人骨髓RPMI-1640,10%胎牛血清27Daudi成淋巴細(xì)胞人淋巴瘤病人血液RPMI-1640,10%胎牛血清28H9成淋巴細(xì)胞人T細(xì)胞淋巴瘤RPMI-1640,20%胎牛血清29HL-60成淋巴細(xì)胞人早幼粒細(xì)胞白血病RPMI-1640,20%胎牛血清30Jurkat成淋巴細(xì)胞人T細(xì)胞白血病RPMI-1640,10%胎牛血清31K-562成淋巴細(xì)胞人骨髓性的白血病RPMI-1640,10%胎牛血清32U937淋巴樣人組織細(xì)胞淋巴瘤RPMI-1640,10%胎牛血清33KG-1骨髓白細(xì)胞人紅白血病病人骨髓IMDM,20%胎牛血清34McCoy成纖維細(xì)胞小鼠未知MEM,10%胎牛血清35BALB/3T3成纖維細(xì)胞小鼠胚胎DMEM,10%胎牛血清363T6成纖維細(xì)胞小鼠胚胎DMEM,10%胎牛血清37A9成纖維細(xì)胞小鼠結(jié)締組織DMEM+10%胎牛血清+6-硫鳥嘌呤（30μg/ml）+2,6-二氨基嘌呤（30μg/ml38AtT-20上皮細(xì)胞小鼠垂體腫瘤F-10,15%馬血清和2.5%胎牛血清39CloneM-3上皮細(xì)胞小鼠黑素瘤F-10,15%馬血清和2.5%胎牛血清40I-10上皮細(xì)胞小鼠睪丸癌F-10,15%馬血清和2.5%胎牛血清41Y-1上皮細(xì)胞小鼠腎上腺瘤F-10,15%馬血清和2.5%胎牛血清42WEHI-3b類巨噬細(xì)胞小鼠骨髓單核細(xì)胞白血病DMEM,10%胎牛血清43ES-D3多能胚胎干細(xì)胞小鼠胚胎型DMEM+15%胎牛血清+100μmol/L2-巰基丙醇44F9胚胎型至內(nèi)胚層小鼠睪丸畸胎癌DMEM,15%胎牛血清45BHK-21成纖維細(xì)胞倉鼠腎GMEM,10%胎牛血清或MEM,10%胎牛血清和NEAA46HaK上皮細(xì)胞倉鼠腎BME,10%小牛血清47CHO-K1上皮細(xì)胞倉鼠卵巢F-12,10%胎牛血清48AR42J大鼠胰腺腫瘤Ham’sF-12K,20%胎牛血清49BRL3A大鼠肝Coon’sF-12K,5%胎牛血清50Clone9上皮細(xì)胞大鼠肝臟F-12K,10%胎牛血清51H4--Ⅱ-E-C3上皮樣大鼠肝癌F-12K,10%胎牛血清52GH1上皮細(xì)胞大鼠垂體腫瘤F-10,15%馬血清和2.5%胎牛血清53GH3上皮細(xì)胞大鼠垂體腫瘤F-10,15%馬血清和2.5%胎牛血清54IEC-6上皮樣大鼠正常小腸DMEM,5%胎牛血清,胰島素0.1μg/ml55L2上皮細(xì)胞大鼠肺F-12K,10%胎牛血清56XC上皮細(xì)胞大鼠肉瘤MEM,10%胎牛血清和NEAA57LLC-WRC256上皮細(xì)胞大鼠癌199培養(yǎng)基,5%馬血清58Jensen成纖維細(xì)胞大鼠肉瘤McCoy’s5A,5%胎牛血清59Rat2(TK-)成纖維細(xì)胞大鼠Rat2(TK-)克隆衍生DMEM,5%胎牛血清,30μg/mlBrdU60PC12NGF誘導(dǎo)的神經(jīng)表型細(xì)胞大鼠腎上腺嗜鉻細(xì)胞RPMI-1640,10%胎牛血清61L6大鼠骨骼肌成肌細(xì)胞DMEM,10%胎牛血清62D-17上皮細(xì)胞狗骨肉瘤MEM,10%胎牛血清和NEAA63BT成纖維細(xì)胞牛鼻甲骨細(xì)胞MEM,10%胎牛血清和NEAA64MARC-145猴猴腎MEM或DMEM,10%小牛血清65CV-1成纖維細(xì)胞猴非洲綠猴腎MEM,10%胎牛血清66COS-1成纖維細(xì)胞猴腎MEM,10%胎牛血清67COS-3成纖維細(xì)胞猴腎MEM,10%胎牛血清68COS-7成纖維