如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座

怎樣探討中藥有效成份對(duì)

基因調(diào)控影響

如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第1頁StimulationInflammatoryproteinSignaltransductionTranscriptionfactorProteinkinasephosphorylationProtein-DNAbindingProteinmRNAAP-1NF-BAP-1NF-BEgr-1MCP-1TF(WesternBlot)*(EMSA)(NorthernBlot)(ELISA)(ex.ERK,P38,JNK)(ex.AP-1,NF-

B,Egr-1)ReceptorGeneRegulationMembraneNuclearex.TNF,IL-1,MCP-1,ICAM-1ex.cytokine,LPS,stressCM98/03/07/94如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第2頁ElectrophoreticMobilityShiftAssay(EMSA)研究DNA結(jié)合蛋白與其相關(guān)結(jié)合序列相互作用方法

Protein/DNA(transcriptionfactor)/(promoterregion)如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第3頁EMSANuclearproteinDNAprobeNativegel(non-denaturingagarosegel)Autoradiography如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第4頁如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第5頁

Promoteractivity

PromoterReportergeneLuciferaseLuciferase

Lucoferin+ATPLight

如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第6頁Regulationofatherosclerosis-relatedgenesbyherbalmedicines

:WoginhibitsMCP-1andTFgeneexpressions如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第7頁P(yáng)rotocolforgeneregulation

?mRNA―NorthernBlot

?mRNAstability―NorthernBlot

?protein―ELISAorWesternBlot

?promoteractivity―Luciferaseactivity

?activationoftranscriptionfactor―EMSA

?signaltransduction―WesternBlot

如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第8頁Atherosclerosis

aprogressivediseasecharacterizedbytheaccumulationoflipidsandfibrouselementsinthelargearteries

?classicconcept:asadisorderoflipidmetabolismanddeposition

?currentview:asachronicinflammatorydisease如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第9頁Threestagesinthelifehistoryofanatheroma

?Initiation:permeability/adhesion/migration

?Progression:fatty-streakformation/advancedlesion

?Complication:plaquesrupture如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第10頁如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第11頁如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第12頁如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第13頁如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第14頁GeneexpressioninthedevelopmentofatherosclerosisInitiationProgressionComplication

MCP-1MMPTFVCAM-1ICAM-1如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第15頁中醫(yī)觀點(diǎn)：《外科正宗》---厚味膏梁,薰蒸臟腑,丹石補(bǔ)藥消灼腎水;房勞過度,氣竭津傷,致使經(jīng)絡(luò)阻塞,氣血凝滯而發(fā)病。屬中醫(yī)“脈痺”和“脫疽”範(fàn)疇血瘀證如何探討中藥有效成分對(duì)基因調(diào)控的影響專家講座第16頁粥狀動(dòng)脈硬化癥：慢性發(fā)炎性疾病

InitiationProgressionComplication

MCP