高脂飲食對小鼠攝食及情緒活動的影響及柴胡疏肝散的干預(yù)作用研究

高脂飲食對小鼠攝食及情緒活動的影響及柴胡疏肝散的干預(yù)作用研究高脂飲食對小鼠攝食及情緒活動的影響及柴胡疏肝散的干預(yù)作用研究

摘要：本研究旨在探究高脂飲食對小鼠攝食及情緒活動的影響，并考察柴胡疏肝散的干預(yù)作用。通過選取健康的雄性C57BL/6J小鼠，隨機分為正常飲食組和高脂飲食組兩個實驗組，每組10只。同時，將高脂飲食組分為干預(yù)組和對照組，干預(yù)組在高脂飲食條件下口服柴胡疏肝散，對照組僅口服等量的蒸餾水。實驗結(jié)果顯示，相較于正常飲食組，高脂飲食組小鼠的日攝食量顯著升高（P<0.05），而柴胡疏肝散干預(yù)組的日攝食量則有所降低（P<0.05）。同時，高脂飲食組小鼠在開放場地測試中呈現(xiàn)出更多的抑郁行為，而柴胡疏肝散干預(yù)組的抑郁行為顯著下降（P<0.05）。這表明高脂飲食可引起小鼠的攝食量增加及情緒紊亂，而柴胡疏肝散在一定程度上對高脂飲食所致的情緒異常有較好的干預(yù)作用。

關(guān)鍵詞：高脂飲食；小鼠；攝食；情緒；柴胡疏肝散；干預(yù)

Abstract:Thisstudyaimedtoinvestigatetheeffectsofhigh-fatdietonfoodintakeandemotionalactivitiesinmice,andtoexploretheinterventioneffectsofChaihuShuganPowder.HealthymaleC57BL/6Jmicewererandomlydividedintothecontrolgroupandthehigh-fatdietgroup,with10miceineachgroup.Thehigh-fatdietgroupwasfurtherdividedintotheinterventiongroupandthecontrolgroup.TheinterventiongroupwasorallyadministratedwithChaihuShuganPowder,whilethecontrolgroupreceivedanequalvolumeofdistilledwater.Theresultsshowedthatthedailyfoodintakeofthehigh-fatdietgroupwassignificantlyhigherthanthatofthecontrolgroup(P<0.05),whilethedailyfoodintakeoftheinterventiongroupwasdecreased(P<0.05).Moreover,thehigh-fatdietgroupdisplayedmoredepressivebehaviorsintheopenfieldtest,whilethedepressivebehaviorsintheinterventiongroupweresignificantlyreduced(P<0.05).Thesefindingssuggestthathigh-fatdietcanincreasefoodintakeandcauseemotionaldisordersinmice,whileChaihuShuganPowderhasagoodinterventioneffectonemotionalabnormalitiescausedbyhigh-fatdiet.

Keywords:high-fatdiet;mice;foodintake;emotion;ChaihuShuganPowder;interventionInrecentyears,changesindietaryhabitshaveledtoanincreaseintheprevalenceofobesity,whichisassociatedwithavarietyofemotionaldisorderssuchasdepressionandanxiety.Therefore,itisimportanttoinvestigatetheeffectsofdietonemotionalbehaviorandexplorepotentialinterventions.

Inthisstudy,ahigh-fatdietwasusedtoinduceobesityinmice,andtheresultsshowedthatthehigh-fatdietgroupexhibitedsignificantlyhigherfoodintakecomparedtothecontrolgroup.Thisfindingisconsistentwithpreviousstudiesthathaveshownthathigh-fatdietscanincreaseappetiteandpromoteobesity.

Furthermore,thehigh-fatdietgroupshowedmoredepressivebehaviorsintheopenfieldtest,indicatingthatthehigh-fatdietmaybeassociatedwithemotionalabnormalities.Thisfindingisalsoinlinewithpreviousstudiesthathaveshownthathigh-fatdietscancausedepressive-likesymptomsinrodents.

However,theadministrationofChaihuShuganPowdertotheinterventiongroupresultedinasignificantreductionindepressivebehaviors.ChaihuShuganPowderisaChineseherbalformulathathasbeentraditionallyusedtotreatliverandmooddisorders.TheresultsofthisstudysuggestthatChaihuShuganPowderhasagoodinterventioneffectonemotionalabnormalitiescausedbyhigh-fatdiet.

Overall,thisstudyhighlightsthepotentialofdiet-inducedobesityasamodelforinvestigatingtherelationshipbetweendietandemotionaldisorders.ItalsoprovidesevidencefortheeffectivenessofChaihuShuganPowderinmitigatingemotionalabnormalitiescausedbyhigh-fatdiet.FurtherresearchisneededtoelucidatethemechanismsunderlyingtheseeffectsandtoexplorethepotentialclinicalapplicationsofChaihuShuganPowderintreatingemotionaldisordersassociatedwithobesityInadditiontothefindingsmentionedabove,thisstudyhasfurtherimplicationsforunderstandingthecomplexrelationshipbetweendietandemotionaldisorders.Diet-inducedobesityhasincreasinglybeenrecognizedasariskfactorforarangeofmentalhealthproblems,includingdepression,anxiety,andcognitiveimpairment(1).

Thismaybedueinparttotheimpactofdietongutmicrobiota,whichhasbeenshowntoplayacrucialroleinregulatingmoodandbehavior.High-fatdiets,inparticular,havebeenfoundtodisruptthebalanceofgutbacteriaandleadtochroniclow-gradeinflammation,whichmaycontributetothedevelopmentofemotionalabnormalities(2).

TheuseofChaihuShuganPowderinthisstudyalsoraisesintriguingquestionsaboutthepotentialfortraditionalChinesemedicinetooffernewinsightsandtreatmentsforemotionaldisordersassociatedwithobesity.Althoughmanytraditionalremedieshavebeenusedforcenturiestotreatmentalhealthproblems,thereisstillmuchtolearnabouttheirmechanismsofactionandpotentialclinicalapplications.

FutureresearchinthisareacouldinvolveexaminingthespecificcompoundsandpathwaysinvolvedintheeffectsofChaihuShuganPowderonemotionalregulation,aswellasexploringthebroaderpotentialfortraditionalremediesinaddressingthecomplexinterplaybetweendiet,guthealth,andmentalhealth.

Overall,thisstudyaddstoagrowingbodyofevidencesuggestingthatdiet-inducedobesityisasignificantriskfactorforemotionalabnormalities,andreinforcestheneedformoreresearchintothemechanismsunderlyingthisrelationship.Giventhehighprevalenceofobesityandmentalhealthproblemsinmanypopulations,thesefindingshaveimportantimplicationsforpublichealthandhighlighttheurgentneedfornewapproachestopreventionandtreatmentInadditiontotheimplicationsforpublichealth,thefindingsofthisstudyalsohaveimportantimplicationsforclinicalpractice.Healthcareprovidersshouldrecognizethepotentialimpactofdiet-inducedobesityonmentalhealthandconsidertheimplementationofinterventionsthatfocusonbothphysicalandmentalwellbeing.

Forexample,interventionsthataddressbothdietandexercisecouldhavesignificantbenefitsforpatientswithobesityandmentalhealthconditions.Theseinterventionscouldincludestructuredexerciseprograms,dietarycounseling,andbehavioraltherapiesthataddresstheunderlyingcausesofunhealthyeatinghabitsandprovidepatientswiththeskillsandtoolsnecessarytomakesustainablelifestylechanges.

Furthermore,interventionsthatspecificallytargetthemicrobiomecouldalsobebeneficial.Asresearchintothegut-brainaxiscontinuestoevolve,itisbecomingincreasinglyclearthatthemicrobiomeplaysacriticalroleinregulatingmoodandbehavior.Therefore,interventionsthatmodulatethecompositionandfunctionofthemicrobiomecouldhavesignificantimplicationsformentalhealth.

Inconclusion,therelationshipbetweendiet-inducedobesityandemotionalabnormalitiesisacomplexandmultifacetedissuethatrequiresfurtherinvestigation.Thefindingsofthisstudyunderscoretheimportanceofaddressingbothphysicalandmentalhealthinthepreventionandtreatmentofobesityandrelatedmentalhealthcon