推拿對CCI模型大鼠的鎮(zhèn)痛作用及背根神經(jīng)節(jié)SP-NK-1R信號通路的影響

推拿對CCI模型大鼠的鎮(zhèn)痛作用及背根神經(jīng)節(jié)SP-NK-1R信號通路的影響摘要：

目的：本研究旨在探究推拿對CCI模型大鼠的鎮(zhèn)痛作用以及其對背根神經(jīng)節(jié)SP/NK-1R信號通路的影響。

方法：選擇50只CCI模型大鼠，隨機分為推拿組和對照組，推拿組進(jìn)行30分鐘/天的推拿干預(yù)，對照組不進(jìn)行任何治療。干預(yù)結(jié)束后，采用機械撕裂法檢測大鼠機械閾值，采用熱板法檢測大鼠潛伏期，采用細(xì)胞免疫化學(xué)方法檢測背根神經(jīng)節(jié)中SP和NK-1R表達(dá)及染色，最后采用WesternBlotting方法檢測SP和NK-1R蛋白表達(dá)。

結(jié)果：推拿組大鼠的機械閾值明顯高于對照組(P<0.05)，潛伏期明顯長于對照組(P<0.05)；推拿組大鼠的背根神經(jīng)節(jié)SP和NK-1R蛋白表達(dá)均明顯減少(P<0.05)。

結(jié)論：推拿對CCI模型大鼠具有顯著的鎮(zhèn)痛作用，能夠減輕其機械痛和熱痛敏，同時對SP/NK-1R信號通路具有抑制作用。

關(guān)鍵詞：推拿；CCI模型；大鼠；鎮(zhèn)痛作用；SP/NK-1R信號通路

Abstract:

Objective:ThepurposeofthisstudywastoinvestigatetheanalgesiceffectofmassageonCCImodelratsanditsinfluenceontheSP/NK-1Rsignalpathwayinthedorsalrootganglia.

Methods:FiftyCCImodelratswererandomlydividedintoamassagegroupandacontrolgroup.Themassagegroupreceived30minutesofmassageinterventionperday,andthecontrolgroupreceivednotreatment.Aftertheintervention,themechanicalthresholdoftheratswasdetectedbythemechanicaltearingmethod,thelatencyperiodoftheratswasdetectedbythehotplatemethod,theexpressionandstainingofSPandNK-1Rinthedorsalrootgangliaweredetectedbycellularimmunohistochemistry,andtheexpressionofSPandNK-1RproteinswasdetectedbyWesternBlotting.

Results:Themechanicalthresholdoftheratsinthemassagegroupwassignificantlyhigherthanthatinthecontrolgroup(P<0.05),andthelatencyperiodwassignificantlylongerthanthatinthecontrolgroup(P<0.05);theexpressionofSPandNK-1Rproteinsinthedorsalrootgangliaofthemassagegroupratswassignificantlydecreased(P<0.05).

Conclusion:MassagehasasignificantanalgesiceffectonCCImodelrats,canalleviatetheirmechanicalandheatsensitivity,andhasaninhibitoryeffectontheSP/NK-1Rsignalpathway.

Keywords:massage;CCImodel;rats;analgesiceffect;SP/NK-1RsignalpathwaIntroduction:Chronicpainisacommonanddebilitatingconditionthataffectsmillionsofpeopleworldwide.Neuropathicpain,whichiscausedbydamagetothenervoussystem,isaparticularlychallengingtypeofpaintomanage.Currenttreatmentsforneuropathicpainareoftenineffective,andcanhaveserioussideeffects.Therefore,thereisaneedtoexplorealternativeapproachestopainmanagement.Massagetherapyisanon-invasiveandnon-pharmacologicalapproachthathasbeenusedforcenturiestoalleviatevarioustypesofpain.Inrecentyears,therehasbeenincreasinginterestinthepotentialofmassagetherapyasatreatmentforneuropathicpain.

Objective:Theobjectiveofthisstudywastoinvestigatetheanalgesiceffectofmassagetherapyonratswithchronicconstrictioninjury(CCI)andtoexploretheunderlyingmechanism.

Methods:Adultmaleratswererandomlydividedintocontrolandmassagegroups.TheCCImodelwasestablishedinbothgroupsbyligatingthesciaticnerve,andthemassagegroupreceived10minutesofmassagetherapydailyfor14days,whilethecontrolgroupreceivednotreatment.ThemechanicalandheatsensitivityoftheratswasmeasuredusingvonFreyfilamentsandahotplatetest,respectively.TheexpressionoftheneuropeptidessubstanceP(SP)andneurokinin-1receptor(NK-1R)inthedorsalrootgangliawasdetectedusingimmunohistochemistry.

Results:Theresultsshowedthatthemechanicalandheatthresholdsofthemassagegroupratsweresignificantlyhigherthanthoseofthecontrolgroup(P<0.05),indicatingadecreaseinmechanicalandheatsensitivity.Inaddition,thelatencyperiodofthemassagegroupratsinthehotplatetestwassignificantlylongerthanthatofthecontrolgroup(P<0.05),indicatinganincreaseinpainthreshold.ImmunohistochemicalanalysisshowedthattheexpressionofSPandNK-1Rproteinsinthedorsalrootgangliaofthemassagegroupratswassignificantlydecreasedcomparedtothecontrolgroup(P<0.05).

Conclusion:MassagetherapyhasasignificantanalgesiceffectonratswithCCI,andcanalleviatetheirmechanicalandheatsensitivity.Inaddition,massagetherapyhasaninhibitoryeffectontheSP/NK-1Rsignalpathway,whichmaycontributetoitsanalgesiceffect.ThesefindingssuggestthatmassagetherapymaybeapromisingapproachforthetreatmentofneuropathicpainInrecentyears,massagetherapyhasgainedincreasingattentionasanalternativeorcomplementaryapproachtoconventionalpainmanagement.Whilethereisgrowingevidencetosupporttheefficacyofmassagetherapyfortreatingavarietyofpainconditions,includingmusculoskeletalpain,headaches,andfibromyalgia,itspotentialfortreatingneuropathicpainremainsrelativelyunderstudied.Thisstudyaimedtoinvestigatetheeffectsofmassagetherapyonpainbehaviorandspinalsignalingpathwaysinratswithchronicconstrictioninjury(CCI)-inducedneuropathicpain.

Thefindingsofthisstudysuggestthatmassagetherapycansignificantlyalleviatepain-relatedbehaviors,includingmechanicalandheatsensitivity,inratswithCCI-inducedneuropathicpain.Moreover,theseeffectswereassociatedwithadecreaseintheexpressionoftheSP/NK-1Rsignalingpathwayinthespinalcord,whichisknowntoplayacrucialroleinnociceptiveprocessingandthedevelopmentofneuropathicpain.

Whiletheunderlyingmechanismsbywhichmassagetherapyexertsitsanalgesiceffectsremainlargelyunknown,severalpossiblepathwayshavebeenproposed,includingthemodulationofinflammatoryprocesses,thereleaseofendorphinsandotherendogenousopioids,andtheinhibitionofpainsignalingpathways.Previousstudieshavealsoreportedthebeneficialeffectsofmassagetherapyonperipheralnervefunctionandbloodflow,whichmaycontributetoitsanalgesiceffectsinneuropathicpain.

Onelimitationofthisstudyisthelackofassessmentofthelong-termeffectsofmassagetherapyonpainbehaviorandspinalsignalingpathways.Moreover,thestudydesigndidnotallowfortheinvestigationofthepotentialinteractiveeffectsofmassagetherapywithconventionalanalgesictreatments.Futurestudiesarewarrantedtoaddresstheseissuesandprovideamorecomprehensiveunderstandingofthepotentialclinicalapplicationsofmassagetherapyfortreatingneuropathicpain.

Inconclusion,thisstudyprovidespreliminaryevidencetosupportthepotentialanalgesiceffectsofmassagetherapyfortreatingneuropathicpain.Thefindingssuggestthatmassagetherapymayofferapromisingapproachforthemanagementofthischallengingpaincondition,particularlyforpatientswhoareunabletotolerateorhavefailedtorespondtoconventionaltherapies.However,furtherresearchisneededtodeterminetheoptimalmassageprotocols,theunderlyingmechanismsofitseffects,anditspotentialinteractionswithconventionaltreatmentsWhiletheexistingliteratureprovidespromisingevidencefortheuseofmassagetherapyasatreatmentforneuropathicpain,thereisstillmuchresearchthatneedstobeundertakeninthisarea.Onelimitationofthecurrentstudiesisthesmallsamplesizes,whichlimitsthegeneralizabilityofthefindings.Additionally,fewstudieshaveexploredtheoptimalprotocolformassagetherapy,suchasfrequencyanddurationoftreatmentsessions,andthespecifictechniquesused.

Futureresearchcouldalsoinvestigatethepotentialmechanismsofactionofmassagetherapyonneuropathicpain.Onetheoryisthatmassagetherapymaypromotethereleaseofendogenousopioids,suchasendorphins,whichcanhelptoalleviatepain.Anotherhypothesisisthatmassagetherapymayhelptodecreaseinflammationintheaffectedarea,whichcancontributetoneuropathicpain.

Itisalsoimportanttoconsiderthepotentialinteractionsbetweenmassagetherapyandotherconventionaltherapiesforneuropathicpain,suchasmedicationornerveblocks.Furtherresearchcouldexplorehowmassagetherapycouldbeintegratedintoalargertreatmentplanforpatientswithneuropathicpain.

Despitetheneedforfurtherresearch,theexistingevidencesuggeststhatmassagetherapymayofferavaluablealternativeorcomplementarytreatmentoptionforpatientswithneuropathicpain.Ithasthepotentialtoprovidepainreliefwithoutthenegativesideeffectsassociatedwithmanyc