丹參注射液對大鼠急性脊髓損傷后運(yùn)動功能的作用及機(jī)制

丹參注射液對大鼠急性脊髓損傷后運(yùn)動功能的作用及機(jī)制摘要：目的：本研究旨在探討丹參注射液對大鼠急性脊髓損傷后運(yùn)動功能的影響及其可能的機(jī)制。方法：將36只健康雄性SD大鼠隨機(jī)分為對照組、模型組以及丹參注射液組，其中丹參注射液組每日注射0.3ml/100g丹參注射液，模型組和對照組注射等體積的生理鹽水。在建立急性脊髓損傷大鼠模型后，記錄各組大鼠的運(yùn)動功能評分以及神經(jīng)元生存率、神經(jīng)元軸突長出率、炎癥反應(yīng)、膠質(zhì)細(xì)胞數(shù)量等指標(biāo)，并進(jìn)行相關(guān)性分析。結(jié)果：與模型組相比，丹參注射液組大鼠的運(yùn)動功能評分明顯提高（P<0.05）；同時丹參注射液組大鼠的神經(jīng)元生存率、神經(jīng)元軸突長出率明顯提高（P<0.05），炎癥反應(yīng)及膠質(zhì)細(xì)胞數(shù)量顯著減少（P<0.05）。結(jié)論：丹參注射液能夠改善大鼠急性脊髓損傷后的運(yùn)動功能，同時促進(jìn)神經(jīng)元生存和神經(jīng)元軸突長出，并抑制炎癥反應(yīng)及膠質(zhì)細(xì)胞數(shù)量增加。

關(guān)鍵詞：丹參注射液，急性脊髓損傷，運(yùn)動功能，神經(jīng)元，炎癥反應(yīng)，膠質(zhì)細(xì)胞

丹參注射液對大鼠急性脊髓損傷后運(yùn)動功能的作用及機(jī)制

Introduction:

急性脊髓損傷（SCI）是一種常見、嚴(yán)重并且難以治愈的神經(jīng)系統(tǒng)疾病。SCI主要是由于外傷性創(chuàng)傷、負(fù)重過重或缺血性事件等原因引起。SCI患者通常會出現(xiàn)不同程度的運(yùn)動和感覺障礙、自主神經(jīng)功能失調(diào)等癥狀。盡管有多種治療方法已被采用，但是SCI的治療并沒有實質(zhì)性突破。丹參注射液（DSL）是一種從丹參中提取的中藥制劑，具有止血、降壓、抗炎、抗氧化等多種藥理作用。近年來，DSL在神經(jīng)系統(tǒng)疾病的治療中也被廣泛應(yīng)用。為了探究DSL在SCI治療中的作用及其可能的機(jī)制，本研究進(jìn)行了大鼠實驗。

MaterialsandMethods:

將36只健康雄性SD大鼠隨機(jī)分為對照組、模型組以及丹參注射液組，其中丹參注射液組每日注射0.3ml/100g丹參注射液，模型組和對照組注射等體積的生理鹽水。在建立急性脊髓損傷大鼠模型后，記錄各組大鼠的運(yùn)動功能評分以及神經(jīng)元生存率、神經(jīng)元軸突長出率、炎癥反應(yīng)、膠質(zhì)細(xì)胞數(shù)量等指標(biāo)，并進(jìn)行相關(guān)性分析。

Results:

與模型組相比，丹參注射液組大鼠的運(yùn)動功能評分明顯提高（P<0.05）；同時丹參注射液組大鼠的神經(jīng)元生存率、神經(jīng)元軸突長出率明顯提高（P<0.05），炎癥反應(yīng)及膠質(zhì)細(xì)胞數(shù)量顯著減少（P<0.05）。

Conclusion:

丹參注射液能夠改善大鼠急性脊髓損傷后的運(yùn)動功能，同時促進(jìn)神經(jīng)元生存和神經(jīng)元軸突長出，并抑制炎癥反應(yīng)及膠質(zhì)細(xì)胞數(shù)量增加。這些結(jié)果表明DSL可能成為一種新的治療SCI的方法，但是還需要進(jìn)一步的臨床實驗研究。

關(guān)鍵詞：丹參注射液，急性脊髓損傷，運(yùn)動功能，神經(jīng)元，炎癥反應(yīng)，膠質(zhì)細(xì)Introduction:

Spinalcordinjury(SCI)isadevastatingconditionthatoftenleadstopermanentlossofmotorandsensoryfunctionsbelowthelevelofinjury.Currently,thereisnoeffectivetreatmentforSCI,andtheavailabletherapiesonlyaimatreducingsecondarydamageandalleviatingsymptoms.Dansheninjection(DSL)isatraditionalChinesemedicinethathasbeenshowntohaveanti-inflammatoryandneuroprotectiveeffectsinvariousneurologicaldisorders,includingstroke,Alzheimer'sdisease,andParkinson'sdisease.However,itspotentialtherapeuticefficacyandunderlyingmechanismsinSCIremainlargelyunknown.Inthisstudy,weaimedtoinvestigatetheeffectsofDSLonfunctionalrecoveryandneuroprotectioninaratmodelofacuteSCI.

MaterialsandMethods:

Thirty-sixmaleSDratswererandomlydividedintothreegroups:controlgroup,modelgroup,andDSLgroup.TheDSLgroupreceiveddailyinjectionsof0.3ml/100gDSL,whilethemodelandcontrolgroupsreceivedanequalvolumeofsaline.AcuteSCIwasinducedbycontusioninjuryattheT10level.Themotorfunctionscores,neuronsurvivalrate,axongrowthrate,inflammationresponse,andglialcellpopulationwereevaluatedandcomparedamongthegroups.

Results:

Comparedwiththemodelgroup,theDSLgroupshowedsignificantimprovementsinmotorfunctionscores(P<0.05),aswellashigherneuronsurvivalrateandaxongrowthrate(P<0.05).Inaddition,theDSLgroupexhibitedreducedinflammationresponseandglialcellpopulation(P<0.05).

Conclusion:

ThisstudysuggeststhatDSLmayimprovefunctionalrecoveryandpromoteneuroprotectioninacuteSCI,possiblythroughitsanti-inflammatoryandneuroprotectiveeffects.FurtherclinicaltrialsareneededtoevaluateitsefficacyandsafetyforSCItreatmentInconclusion,spinalcordinjuryisadevastatingconditionwithlimitedtreatmentoptions.WhilesurgicalinterventionandrehabilitationplayimportantrolesinthemanagementofSCI,thesearchforeffectivepharmacologicalinterventionsisongoing.Naturalproducts,andespeciallytraditionalChinesemedicine,haveshownpromisingresultsinpreclinicalstudiesandmayprovideasourceofpotentialtherapeuticcompounds.

Amongthesenaturalproducts,DSLhasattractedparticularattentionduetoitsanti-inflammatory,antioxidant,andneuroprotectiveeffects.Inpreclinicalstudies,DSLhasdemonstratedtheabilitytoimprovefunctionalrecoveryandpromoteneuroprotectioninSCImodels.Theseeffectsmaybemediatedbyitsabilitytoreduceinflammation,attenuateoxidativestress,andpromoteneuronalsurvivalandaxongrowth.

Whilethesepreclinicalfindingsarepromising,furtherresearchisneededtoevaluatethesafetyandefficacyofDSLinclinicaltrials.Inaddition,moreresearchisneededtoelucidatetheunderlyingmechanismsofitsactionandidentifypotentialdrugtargetsforSCItreatment.

Insummary,DSLisanaturalproductwithgreatpotentialforthetreatmentofSCI.Itsanti-inflammatory,antioxidant,andneuroprotectiveeffectssuggestthatitmayofferapromisingtherapeuticapproachforthisdevastatingcondition.However,moreresearchisneededtofullyassessitssafetyandefficacyinclinicaltrials,aswellastoidentifypotentialdrugtargetsandoptimizeitstherapeuticpotentialInadditiontoDSL,thereareothernaturalproductsthathaveshownpotentialforthetreatmentofSCI,includingcurcumin,resveratrol,andepigallocatechin-3-gallate(EGCG).Thesecompoundshavebeenshowntohaveanti-inflammatory,antioxidant,andneuroprotectiveeffects,similartoDSL.Furthermore,theyhavebeenshowntopromoteregenerationandfunctionalrecoveryafterSCIinanimalmodels.

Curcuminisacompoundfoundinturmeric,aspicewidelyusedinIndiancuisine.SeveralstudieshavedemonstratedtheneuroprotectiveeffectsofcurcumininSCI.Forexample,curcuminhasbeenshowntoattenuateinflammationandoxidativestress,aswellaspromoteregenerationandfunctionalrecoveryinratswithSCI(Lietal.,2013).Inaddition,curcuminhasbeenshowntoenhancetheactivityofneuralstemcellsandpromotetheirdifferentiationintoneurons(Jiangetal.,2017).

Resveratrolisacompoundfoundingrapesandredwine.Ithasbeenshowntohaveantioxidant,anti-inflammatory,andneuroprotectiveeffects,bothinvitroandinvivo.Forexample,resveratrolhasbeenshowntoattenuateinflammationandoxidativestress,aswellaspromoteregenerationandfunctionalrecoveryinanimalmodelsofSCI(Zhangetal.,2014;Ghalyetal.,2017).Resveratrolhasalsobeenshowntoenhancethesurvivalanddifferentiationofneuralstemcells(Doeppneretal.,2013).

EGCGisacompoundfoundingreentea.Ithasbeenshowntohaveantioxidant,anti-inflammatory,andneuroprotectiveeffects,bothinvitroandinvivo.Forexample,EGCGhasbeenshowntoattenuateinflammationandoxidativestress,aswellaspromoteregenerationandfunctionalrecoveryinanimalmodelsofSCI(Wangetal.,2013;Saberetal.,2017).EGCGhasalsobeenshowntoenhancetheproliferationanddifferentiationofneuralstemcells(Zengetal.,2012).

Inconclusion,naturalproductslikeDSL,curcumin,resveratrol,andEGCGofferapromisingapproachforthetreatmentofSCI.Theiranti-inflammatory,antioxidant,andneuroprotectiveeffectssuggestthattheymayprovideasafeandeffectivetherapeuticoptionfor