PFC腦區(qū)促進(jìn)睡眠神經(jīng)元

CorrespondingAuthorYangDan,PhDBS,physics,BeijingUniversityPhD,neurobiology,ColumbiaUniversityCurrentpositionsHowardHughesMedicalInstituteUniversityofCalifornia,Berkeley

CurrentprojectsNeuralcircuitscontrollingsleep.Functionoftheprefrontalcortex(PFC).當(dāng)前1頁，總共21頁。BackgroundDifferentwavesgalleryhip/rem-sleep-cycle.html當(dāng)前2頁，總共21頁。BackgroundSleepcyclegalleryhip/rem-sleep-cycle.html當(dāng)前3頁，總共21頁。BackgroundPOA

Lesions

inthePOAcausesleepimpairment.Sleep-activeneuronsexistinthePOA.Thesleep-activeneuronsarespatiallyintermingled

withwake-activeneurons.Preopticarea,aregionofthehypothalamusmedialpreopticnucleusmedianpreopticnucleuslateralpreopticnucleusperiventricularpreopticnucleus/wiki/Preoptic_area當(dāng)前4頁，總共21頁。BackgroundTMNTuberomammillarynucleus,aregionoftheposteriorhypothalamuslargely,wake-promotinghistaminergicneuronsarousal,learning,memory,sleepandenergybalance./wiki/Tuberomammillary_nucleus當(dāng)前5頁，總共21頁。GABAPOA→TMNExtendedDataFigure1a當(dāng)前6頁，總共21頁。GABAPOA→TMNGABAergicneuronshistaminergicneuronsPOATMNGABAPOA→TMNExtendedDataFigure1b當(dāng)前7頁，總共21頁。GABAPOA→TMN

singlesoutthesleep-promotingneuronsGABAPOA→TMN96.2±1.4%95.4±3.7%ExtendedDataFigure2h當(dāng)前8頁，總共21頁。GABAPOA→TMN

singlesoutthesleep-promotingneuronsGABAPOA→TMN73.6±4.8%SleepreboundGABAergicneuronshistaminergicneuronsPOATMNGABAPOA→TMNSleep-promotingExtendedDataFigure2i當(dāng)前9頁，總共21頁。TwopartsoftheessayIdentificationofpreopticsleepneuronsusingretrogradelabellingandgeneprofiling當(dāng)前10頁，總共21頁。RetrogradelabellingCausalrole:OptogeneticactivationorinhibitionofGABAPOA→TMNneuronsenhancesorsuppressessleep.Figure1Extendeddatafigure3當(dāng)前11頁，總共21頁。RetrogradelabellingDataanalysisActivationInhibitionControlExtendeddatafigure5當(dāng)前12頁，總共21頁。RetrogradelabellingFunctionallydistinct:OptogeneticactivationofGABAPOAorVGLUTPOA→TMNneuronspromoteswakefulness.Figure3KEY1:TMNprojectingKEY2:GABAergic當(dāng)前13頁，總共21頁。RetrogradelabellingOptrode:MeasuredirectlythespikingactivityofChR2-labelledGABAPOA→TMNneurons.Figure4當(dāng)前14頁，總共21頁。RetrogradelabellingOptrode:MeasuredirectlythespikingactivityofChR2-labelledGABAPOA→TMNneurons.17identifiedGABAPOA→TMNneurons51unidentifiedPOAunitsFigure3當(dāng)前15頁，總共21頁。GeneprofilingTRAPTranslatingRibosomeAffinityPurificationFigure4aRNA-seq當(dāng)前16頁，總共21頁。GeneprofilingCCKCRHFigure4bcTRAP(cholecystokinin)(corticotropin-releasinghormone)當(dāng)前17頁，總共21頁。GeneprofilingCCKCRHExtendeddatafigure10當(dāng)前18頁，總共21頁。GeneprofilingRNA-seqTAC1(Preprotachy-kinin-1)&PDYN(ProDynorpy-in)當(dāng)前19頁，總共21頁。ConclusionUsingoptogeneticmanipulationandrecording,virus-mediatedcircuittracing,andgeneprofiling,wehavecharacterizedapopulationofPOAsleepn