CORM-401-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemECORM-401Cat.No.:HY-109804CASNo.:1001015-18-4分?式:C?H?MnNO?S?分?量:331.2作?靶點:ReactiveOxygenSpecies作?通路:Immunology/Inflammation;MetabolicEnzyme/Protease;NF-κB儲存?式:Powder-20°C3yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實驗DMSO:25mg/mL(75.48mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM3.0193mL15.0966mL30.1932mL5mM0.6039mL3.0193mL6.0386mL10mM0.3019mL1.5097mL3.0193mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內(nèi)使?，-20°C儲存時，請在1個?內(nèi)使?。體內(nèi)實驗請根據(jù)您的實驗動物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結(jié)果的可靠性，澄的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:2.5mg/mL(7.55mM);Suspendedsolution;Needultrasonic2.請依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESolubility:≥2.5mg/mL(7.55mM);ClearsolutionBIOLOGICALACTIVITY?物活性CORM-401?種對氧化劑敏感的CO釋放分?。CORM-401誘導(dǎo)內(nèi)?鈣信號調(diào)節(jié)中的NO增加。CORM-401減少TNF-α/CHX和H2O2誘導(dǎo)的ROS產(chǎn)?。CORM-401解耦線粒體呼吸并抑制糖酵解[1][2][3]。體外研究CORM-401(100μM;1h)inducestheNOproductioninendothelialEA.hy926cells[1].CORM-401(30μM)inducespeak-likecalciumsignalandaugmentescouplingofendoplasmicreticulumandplasmalemmalstore-operatedcalciumchannels[1].CORM-401(50μM;1h)significantlyreducesTNF-α/CHXandH2O2-inducedROSproductionandcelldeath[2].CORM-401(0.5,1mM)inducesapersistentincreaseintheoxygenconsumptionrateinendothelialEA.hy926cells[3].CORM-401(10,30,100μM)inducesaconcentration-dependentincreaseinOCRandasimultaneousdecreaseinECAR[3].REFERENCES[1].KaczaraP,etal.CORM-401inducescalciumsignalling,NOincreaseandactivationofpentosephosphatepathwayinendothelialcells.FEBSJ.2018Apr;285(7):1346-1358.[2].BabuD,etal.DifferentialEffectsofCORM-2andCORM-401inMurineIntestinalEpithelialMODE-KCellsunderOxidativeStress.FrontPharmacol.2017Feb8;8:31.[3].KaczaraP,etal.CarbonmonoxidereleasedbyCORM-401uncouplesmitochondrialrespirationandinhibitsglycolysisinendothelialcells:AroleformitoBKCachannels.BiochimBiophysActa.2015Oct;1847(10):1297-309.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forre