IMB-301-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEIMB-301Cat.No.:HY-122156CASNo.:64009-84-3分?式:C??H??Cl?FN?O分?量:379.26作?靶點(diǎn):HIV作?通路:Anti-infection儲(chǔ)存?式:Pureform-20°C3yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:100mg/mL(263.67mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.6367mL13.1836mL26.3671mL5mM0.5273mL2.6367mL5.2734mL10mM0.2637mL1.3184mL2.6367mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(6.59mM);Clearsolution2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESolubility:≥2.5mg/mL(6.59mM);Clearsolution3.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(6.59mM);ClearsolutionBIOLOGICALACTIVITY?物活性IMB-301?種特異性的HIV-1復(fù)制抑制劑，能與hA3G(humanAPOBEC3G)結(jié)合，阻斷hA3G-Vif相互作?，抑制Vif介導(dǎo)的hA3G降解。IMB-301抑制H9細(xì)胞中HIV-1的復(fù)制(IC50=8.63uM)。?APOBEC3G?種抑制HIV-1復(fù)制的限制因?[1]。體外研究IMB-301isabletoincreasevirionencapsidationofhA3GviaitsabilitytoimpairthedegradationofhA3GbyVif,whichresultsinhA3G-dependentanti-HIVactivity.IMB-301bindstohA3G,restoreshA3GexpressioninthepresenceofVif,andinhibitsthereplicationofHIV-1inahA3G-dependentmanner[1].REFERENCES[1].MaL,etal.IdentificationofsmallmoleculecompoundstargetingtheinteractionofHIV-1VifandhumanAPOBEC3Gbyvirtualscreeningandbiologicalevaluation.SciRep.2018;8(1):8067.Published2018May23.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820