Ruthenium-red-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?Agonists?ScreeningLibrariesRutheniumredCat.No.:HY-103311CASNo.:11103-72-3作?靶點(diǎn):CalciumChannel作?通路:MembraneTransporter/IonChannel;NeuronalSignaling儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體DMSO:10mg/mL(Needultrasonic)外H2O:<0.1mg/mL(ultrasonic)(insoluble)實(shí)請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制驗(yàn)儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存?式和期限。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InV制澄的儲(chǔ)備液，再依次添加助溶劑：為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶1.請(qǐng)依序添加每種溶劑：10%DMSO90%(20%SBE-β-CDinsaline)Solubility:1mg/mL(InfinitymM);Clearsolution;Needultrasonic此?案可獲得1mg/mL(InfinitymM)的澄溶液。以1mL?作液為例，取100μL10.0mg/mL的澄DMSO儲(chǔ)備液加到900μL20%的?理鹽??溶液中，混合均勻。BIOLOGICALACTIVITY?物活性Rutheniumred(Ammoniatedrutheniumoxychloride)?種聚陽(yáng)離?染料，?泛?于電?顯微鏡(EM)下細(xì)胞、組織和營(yíng)養(yǎng)細(xì)的觀察。Rutheniumred能與磷脂和脂酸反應(yīng)強(qiáng)烈，并與酸性粘多糖結(jié)合。Rutheniumred還?種L型鈣電流(ICa)阻斷劑[1][2]。IC50&TargetICa[2]1/2體外研究Rutheniumredstainingofintracellularorganellesandstructuresseemedtodependonthetimeofexposuretthisagent,aswellontheconcentrationused[2].RutheniumredefficientlyblockstheL-typecalciumcurrentinadose-dependentmannerininguinea-pigisolatedventricularheartcells,withEC50of0.8μM[2].Rutheniumred(10μM)blocksthesarcoplasmicCareleasechannelsorthemitochondrialCauptake,andblocks26.7%ofthesodiumcurrent,andsloweditsinactivationtime-courseinguinea-pigisolatedventricularheartcells[2].REFERENCES[1].LashandaNWaller,etal.RutheniumredstainingforultrastructuralvisualizationofaglycoproteinlayersurroundingthesporeofBacilluanthracisandBacillussubtilis.JMicrobiolMethods.2004Jul;58(1):23-30.[2].ClaireOMalécot,etal.Rutheniumredasaneffectiveblockerofcalciumandsodiumcurrentsinguinea-pigisolatedventricularheartcells.BrJPharmacol.1998Jun;124(3):465–472.McePdfHeightMedChemExpress(MCE)只為有資質(zhì)的科研機(jī)構(gòu)、醫(yī)藥企業(yè)基于科學(xué)研究或藥證申報(bào)的?途