Sulforhodamine-B-sodium-salt-Acid-Red-52-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemESulforhodamine B sodium saltCat. No.: HY-D0974CAS No.: 3520-42-1Synonyms: Acid Red 52; Kiton Red 620分式: CHNNaOS分量: 580.65作靶點(diǎn): Others作通路: Others儲(chǔ)存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect from

2、light)溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 50 mg/mL (86.11 mM; Need ultrasonic)H2O : 20 mg/mL (34.44 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 1.7222 mL 8.6110 mL 17.2221 mL5 mM 0.3444 mL 1.7222 mL 3.4444 mL10 mM 0.1722 mL 0.8611 mL 1.7222 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。BIOLO

3、GICAL ACTIVITY物活性 Sulforhodamine B sodium salt種熒光染料，可于定量測(cè)定培養(yǎng)細(xì)胞的細(xì)胞蛋量。體外研究Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell densitydetermination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used toinexpensively conduct v

4、arious screening assays to investigate cytotoxicity in cell based studies. This methodrelies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEcan be extracted using basic conditions; thus, the amo

5、unt of bound dye can be used as a proxy for cell mass,which can then be extrapolated to measure cell proliferation. The protocol can be divided into four mainsteps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining,and absorbance measurement. This

6、 assay is limited to manual or semiautomatic screening, and can be usedin an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. Italso has applications in evaluating the effects of gene expression modulation (knockdown, gene expressionupregulation), a

7、s well as to study the effects of miRNA replacement on cell proliferation 1.PROTOCOLCell Assay 1 Gently add 25 L (96-well format) or 5 L (384-well format) cold 50% (wt/vol) TCA to each well directly tomedium supernatant, and incubate the plates at 4 C for 1 h. Mixing is not required, as this could l

8、ead tosome cells detaching from the bottom of the well. Wash the plates four times by submerging the plate in a tubwith slow-running tap water and remove excess water by gently tapping the plate into a paper towel. After thelast wash allow the plate to air-dry at room temperature. Add 50 L (96-well

9、format) or 20 L (384-wellformat) of 0.04% (wt/vol) SRB solution to each well. Leave at room temperature for 1 h and then quickly rinsethe plates four times with 1% (vol/vol) acetic acid (200 L for 96-well format or 30 L for 384-well format) toremove unbound dye. Allow the plate to air-dry at room te

10、mperature 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii:e1984.McePdfHeightCaution: Product has not been fully valida