CMK - Ribosomal S6 Kinase (RSK) 抑制劑 - 生命科學(xué)試劑 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemECMKCat. No.: HY-52101CAS No.: 821794-90-5分式: CHClNO分量: 358.82作靶點: Ribosomal S6 Kinase (RSK)作通路: MAPK/ERK Pathway儲存式: 4C, stored under nitrogen* In solvent : -80C, 6 months; -20C, 1 month (stored undernitrogen)溶解性數(shù)據(jù)體外實驗 DMSO : 15

2、0 mg/mL (418.04 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 2.7869 mL 13.9346 mL 27.8691 mL5 mM 0.5574 mL 2.7869 mL 5.5738 mL10 mM 0.2787 mL 1.3935 mL 2.7869 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。體內(nèi)實驗 請根據(jù)您的實驗動物和給藥式選擇適當(dāng)?shù)娜芙獍?，配制前請先配制澄清的儲備液，再依次添加助溶?為保證實驗結(jié)果的可靠性，體內(nèi)實驗的作液

3、，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (6.97 mM); Clear solution2. 請依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (6.97 mM); Clear solutionBIOLOGICAL ACTIVITY1/2 Master of Small Molecules 您邊的抑制劑師

4、www.MedChemE物活性 CMKRSK2激酶抑制劑，與FMK相，具有相似的效但化學(xué)穩(wěn)定性較低。體外研究 CMK inhibits the growth of Cdc5 (L158G) with IC50 of 36 nM, greater than 30 M for wild type Cdc5. CMKexhibits a concentration-dependent first cell cycle mitotic arrest in the cdc5-as1 strain with an IC50 of 1.1 M.CMK inhibition of Cdc5 (L158G) l

5、eads to a first cell cycle anaphase arrest and delay in anaphase spindlemigration 1.PROTOCOLKinase Assay 1 For Cdc5 kinase assays, Spc72-TAP is isolated from cell extract by pulldown with IgG sepharose or rabbitIgG coupled to M-270 epoxy dynabeads and incubated in kinase buffer (25 mM HEPES, pH 8.0,

6、 60 mM KCl,15 mM MnCl2, 100 g/mL BSA, 80 nM microcystin, 1mM DTT, 100 M 200 Ci/mL -32PATP) in theabsence or presence of 100 ng purified baculovirus expressed 6xHis-Cdc5. 32P incorporation is visualizedon a Typhoon PhosphorImager, and images are processed using ImageQuant software.MCE has not indepen

7、dently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Cell Chem Biol. 2018 Feb 15;25(2):154-165.e11.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Snead JL, et al. A coupled chemical-genetic and bioinformatic approach to Polo-like kinase pathway exploration. Chem Biol. 2007Nov;14(11):1261-72.McePdfHeightCaution: Product has not been fully validated for medical applications.For researc