Engeletin - NF-κB 抑制劑 - 生命科學試劑 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEEngeletinCat. No.: HY-N0436CAS No.: 572-31-6分式: CHO分量: 434.39作靶點: NF-B作通路: NF-B儲存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實驗 DMSO : 83.3 mg/mL (191.76 mM)* means soluble, but saturation unknown.

2、Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 2.3021 mL 11.5104 mL 23.0208 mL5 mM 0.4604 mL 2.3021 mL 4.6042 mL10 mM 0.2302 mL 1.1510 mL 2.3021 mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Engeletin是從 hymenaea martiana 中得到的黃酮苷類物質(zhì)，能夠抑制 NF-B 信號通路的激活，具有抗炎、鎮(zhèn)痛、利尿、消腫、抗 等作。IC50

3、 & Target NF-B體外研究Engeletin is a flavanonol glycoside isolated from hymenaea martiana, inhibits NF-B signaling-pathway1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEactivation 1.體內(nèi)研究 Engeletin (25, 50, 100 mg/kg, i.p.) markedly reduces LPS-increased myeloperoxidase activity in mice,activates NF-B-

4、pathway activation, decreases the production of inflammatory mediators (iNOS and COX-2),and suppresses the expression of TLR4-signaling downstream molecules such as MyD88, IRAK1, TRAF6,and TAK1 proteins 1.PROTOCOLAnimal Mice 1Administration 1 The mice are classified at random into the following six

5、groups of 10 mice each to cause the endometritismodel: blank group, LPS group, Engeletin (25, 50, and 100 mg/kg) + LPS groups, and Engeletin (100 mg/kg)group. Engeletin is solubilized by heated normal saline to give the final concentrations of 25, 50, and 100mg/kg. Briefly, each uterus is infused wi

6、th 50 L of LPS (1 mg/mL) to induce endometritis. At 24 h after theinstillation, Engeletin groups receive an intraperitoneal injection of diverse Engeletin concentrations (25, 50,and 100 mg/kg) three times (once every 6 h). The Engeletin group is given an intraperitoneal injection ofEngeletin (100 mg

7、/kg). The blank group receive the normal saline. Afterward, the mice are killed by CO2inhalation. Uterus tissues are harvested and kept in 80C 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Wu H, et al. Engeletin Alleviates Lipopolysaccharide-Induced Endometritis in Mice by Inhibiting TLR4-mediated NF-B Activation. J AgricFood Chem. 2016 Aug 10;64(31):6171-8.McePdfHeightCaution: Product has not been fully validated for medical applications.For rese