丁香酚作用機(jī)制 - Medchemexpress - MCE中國(guó)

1、Product Data SheetEugenolCat. No.: HY-N0337CAS No.: 97-53-0分式: CHO分量: 164.2作靶點(diǎn): Bacterial; Parasite; Reactive Oxygen Species; Apoptosis; Ferroptosis作通路: Anti-infection; Immunology/Inflammation; Metabolic Enzyme/Protease; NF-B;Apoptosis儲(chǔ)存式: Pure form -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C

2、 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 100 mg/mL (609.01 mM)* means soluble, but saturation unknown.SolventMass1 mg 5 mg 10 mgConcentration制備儲(chǔ)備液1 mM 6.0901 mL 30.4507 mL 60.9013 mL5 mM 1.2180 mL 6.0901 mL 12.1803 mL10 mM 0.6090 mL 3.0451 mL 6.0901 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存式和期限

3、：-80C, 6 months; -20C, 1 month。-80C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)內(nèi)使，-20C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)內(nèi)使。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍浮Ｒ韵氯芙獍付颊?qǐng)先按照 In Vitro 式配制澄清的儲(chǔ)備液，再依次添加助溶劑：為保證實(shí)驗(yàn)結(jié)果的可靠性，澄 的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使； 以下溶劑前顯的百分 指該溶劑在您配制終溶液中的體積占；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的式助溶1. 請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineS

4、olubility: 3.25 mg/mL (19.79 mM); Clear solution此案可獲得 3.25 mg/mL (19.79 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 32.5 mg/mL 的澄 DMSO 儲(chǔ)備液加到 400 L PEG300 中，混合均勻；向上述體系中加50 L Tween-80，混合均勻；然后繼續(xù)加 450 L 理鹽定容 1 mL。2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 3.25 mg/mL (19.79 mM); Clear solutionP

5、age 1 of 2 www.MedChemE此案可獲得 3.25 mg/mL (19.79 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L 32.5 mg/mL 的澄 DMSO 儲(chǔ)備液加到 900 L 20% 的 SBE-CD 理鹽溶液中，混合均勻。3. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 3.25 mg/mL (19.79 mM); Clear solution此案可獲得 3.25 mg/mL (19.79 mM，飽和度未知) 的澄 溶液，此案不適于實(shí)驗(yàn)周 期在半個(gè)以上的實(shí)驗(yàn)。以 1 mL 作液為例，取 100 L

6、 32.5 mg/mL 的澄 DMSO 儲(chǔ)備液加到 900 L 油中，混合均勻。BIOLOGICAL ACTIVITY物活性 Eugenol丁中發(fā)現(xiàn)的精油，具有抗 ，驅(qū)和抗氧化活性。 Eugenol也可抑制脂質(zhì)過(guò)氧化。IC & Target Bacterial, Parasite1體外研究 The essential oil of O. gratissimum, as well as eugenol, are efficient in inhibiting eclodibility of H. contortus eggs,showing possible utilizations in th

7、e treatment of gastrointestinal helmintosis of small ruminants. At 0.50%concentration, the essential oil and eugenol show a maximum eclodibility inhibition1. Eugenol inhibits superoxideanion generation in xanthine-xanthine oxidase system to an extent of 50% at concentrations of 250 M. Eugenol alsoin

8、hibits the generation of hydroxyl radicals to an extent of 70%. The OH-radical formation measured by thehydroxylation of salicylate to 2, 3-dihydroxy benzoate is inhibited to an extent of 46% by eugenol at 250 M2.Eugenol protects against RS-induced development of IBS-like gastrointestinal dysfunctio

9、n through modulation ofHPA-axis and brain monoaminergic pathways apart from its antioxidant effect. Eugenol (50 mg/kg) reduces 80% ofRS-induced increase in fecal pellets similar to that of ondansetron. Eugenol attenuates 80% of stress-induced increasein plasma corticosterone and modulates the seroto

10、nergic system in the PFC and amygdala. Eugenol attenuates stress-induced changes in norepinephrine and potentiates the antioxidant defense system in all brain regions3.體內(nèi)研究 Eugenol (33 mg/kg) administered orally for 2 days causes significant suppression of knee joint edema, whichcontinues to be sign

11、ificantly reduced at the end of the treatment. After 2 days, eugenol-treated mycobacterialarthritic rats show a marked reduction in paw swelling4.PROTOCOLCell Assay 1 The essential oil and eugenol are diluted in aqueous solution of Tween 20 (0.5%) in the following concentrations:0.0625, 0.12, 0.25,

12、0.5 and 1.0% to be used in the egg hatch test1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Rats: The treatment groups of arthritic rats are given either ingwerol (0.33 mL/kg or 33 mg/kg) or eugenol (0.33Administration 4 mL/kg or 33 mg/kg) ora

13、lly 1 day prior to the induction of arthritis. This treatment is continued for 26 days on a dailybasis. Mycobaterium trated rats receive physiological saline orally4.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Pessoa LM, et al. Anthelmi

14、ntic activity of essential oil of Ocimum gratissimum Linn. and eugenol against Haemonchus contortus. Vet Parasitol. 2002 Oct16;109(1-2):59-63.Page 2 of 3 www.MedChemE2. Reddy AC, et al. Studies on the inhibitory effects of curcumin and eugenol on the formation of reactive oxygenspecies and the oxida

15、tion of ferrous iron.Mol Cell Biochem. 1994 Aug 17;137(1):1-8.3. Garabadu D, et al. Protective effect of eugenol against restraint stress-induced gastrointestinal dysfunction: Potential use in irritable bowel syndrome.Pharm Biol. 2015 Jul;53(7):968-74.4. Sharma JN, et al. Suppressive effects of eugenol and ginger oil on arthritic rats. Pharmacology. 1994 Nov;49(5)