3日王歡博水中有毒有害污染物的三維熒光光譜特性分析.

1、11Characterization study of the toxicorganic pollutants in water byexcitation-emission matrixfluorescenceAnhui Institute of Optics and Fine Mechanics WangHuanboMContents Introduction MethodResults And DiscussionConclusion and ProspectbsgggaMpa_lETIntroductionco cS C&OQOa?0co? o5o coSChrysene Bnz

2、o(a)anthracene Benzo(b)fluoranthene Benzo(k)fluoranthene IntroductionHPLCGCGC-MSthe equipment are expensivelarge amounts of hazardous organic solvents tedious andtime-consuming pretreatment proydur”IContents Introduction Object MethodResults And DiscussionConclusion and ProspectLET -亠 J= TL一Objectch

3、aracteristics study of pure BKF and Per byexcitati on-emissio n matrix fluoresce nee (EEM)Quantitative analysis of BKF-Per mixtures by EEMcoupled with PARAFAC algorithemIntroductionBenzo(k)fluoraContentsObjectMethodResults And DiscussionConclusion and Prospect Mothod-excitation-emission fluorescence

4、ContentsIntroductionObjectMethod: Results And DiscussionConclusion and ProspectEquipmentHITACHI F-7000SKALAR FLUO-IMAGER MONITOR M153LETLETResults-Excitation-emission matrix spectraResults-Excitation-emission matrix spectrapeak regions（EX/EM）252nm/438nm252nm/466nmuorlJoxuj300150400450500Emission wav

5、e*ngth（nrn）Three-dime nsional plotContourspectraBKFpeak regions（EX/EM）246nm/406nm246nrrV432nm306nm；406nm306n m/432nmperyleneEmssjonwavolonalhResults-excitation and emission spectraBKFFluorescence spectra of two substances existin the phenomenon of Stokes shiftResults-qualitative analysisPerLETALy5ul

6、Jlnlwa6laKnlJ2丄EmMioror1rf.1a創(chuàng)NU04IUIVEThe spectra of mixture sampleRD”o3rM1uRUKJXUCUJIM殳uuqfuMlLJOO 350400450 BOOEmission343W2920(EUr&UQtvAeMucnsux山Chemometricsalgorithms-Tri-linear component modelsxi.k=y?aififckf+孤IneludetwoA isexcitationAlternatiChemometrics algorithms-PARAFACFigures of merit

7、RMSEP-if *REP-VNSEL噸(BYB)T(ATA)JJ2SENJ(BTB)T(ATA)jn嚴Results-Linear rangeBKFS$ MOPeak valueex/emRegression equationcorrelationcoefficientLinear rangeBKF306/i06nm100.31741-2.93720. 999610816y g/LPer252/438nr=173. 88156xU2. 138010. 999830.6-20M g/LResultsresolved spectra| Both of the excitation and emi

8、ssion spectra are overlappedResults-recoveryall the predict!on concentration are close to the standard one Therecovery of each sample are close to 100%Resultsfigures of meritRecovery(%)RMSEP(mg/L)REP(%)SELSENBKF101.020.09071.7990.4529589.15Per99.510.02150.6900.2280318.86A good performance has been a

9、chieved usingexcitation-emission fluorescence coupled withPARAFAC algorithm ContentsIntroductionObjectMethodResults And DiscussionConclusion and ProspectConclusion and ProspectQualitative analysis:eachPAHhas a Characteristicfluoresce nee spectrumQuantitative analysis:excitation-emission matrices have perfect tri-linearproperty, PARAFAC algorithm was used to resolve theproblem of spectra overlapping and obtain the predictionconcentration of the unknown sampleFigures ofmeritwas considered to assess the performanee ofPARAFAC algorithm.