響應(yīng)面優(yōu)化法對香菇多糖提取的工藝研究_圖文

1、 14遼寧農(nóng)業(yè)科學(xué)2010年 圖5Y=f(A,B的響應(yīng)面圖和等高線圖Figure 5R皓ponsiVe surfac鶴andntou瑙of Y=f(A.B 圖6Y=f(A。c的響應(yīng)面圖和等高線圖Figure 6R唧。嘣ve鋤rfaces ad咖tours of Y=f(A。C1 圖7Y=f(B,c的響應(yīng)面圖和等高線圖Figre 7Responsive surfhces andcotours of Y=f(B.C圖5顯示了A由高水平到低水平變化過程中等高線的數(shù)目明顯多于B,說明浸提溫度對香菇多糖得率的影響很顯著。由圖6可以看出,等高線呈橢圓形,且橢圓的軸線與坐標(biāo)軸之間的有較大的角度,說明溫度與料

2、液比之間存在顯著的交互作用。對多元二次回歸方程進行求偏導(dǎo),可以得到:l,=1.060.013曰一6.5A+0.84C:l,=O.19一O.013A一0.42口+0.73C;y=0.60+O.84A+O.73日一3.92C聯(lián)立方程組,解得熱水浸提香菇多糖最優(yōu)條件的編碼值如下:A=0.138,B=0.002,C=0.169。即在浸提溫度為78.6,浸提時間為120min,料液比為l:58.3時,模型預(yù)測香菇粗多糖得率最大值為14.12%。3結(jié)論通過BoxBenhnken設(shè)計和響應(yīng)面分析法,利用DesignExperc統(tǒng)計軟件能夠可靠地進行優(yōu)化實驗以及數(shù)據(jù)處理。本實驗應(yīng)用這一方法對香菇多糖提取的工藝

3、條件進行了優(yōu)化。在單因素實驗的基礎(chǔ)上,應(yīng)用BoxBenhnken設(shè)計三因素三水平的實驗,確定出香菇多糖提取的優(yōu)化工藝條件為:提取溫度78.6,提取時間120min和料液比1:58.3,提取得率為14.22%及其多糖含量為38.57%。參考文獻:Ch蛐g(shù)sT.Mush刪biology:theimp虬t口nmuBhroomp一duction蛆dmushmomproductBJ.Mu8hfoom Biology andMu8hroomProduct8.1993.122:320.王志明,陳祥貴.香菇營養(yǎng)成分的酶解提取及利用J.四JII工業(yè)學(xué)院學(xué)報,1998,17(1:4649.ZHOULD,zHANG

4、QH,ZHANGY.The 8hiitake mushrmderived immune-8timulant lentinanprot即ts醒ainstmurinema-ldablood-暑tage infec“onby evoking adaptive immune一咒Bpon-啷J.Int JImmunopha咖acol,2009,9(4:455462.Chiham G。Maeda YY,H8mur0J。SB8aki T,FumikoF.Inhibitionof mouseBarcomaI 80bypolysacchade8fmmI七ntinuBedode8J.Nalure,1969,222

5、:687688.Fue H,Kjtoh I.Ph粕e ll l一8tudy onLentinan.J印蛐e8eJ.CcerChemotheMpy。1981.8:944960.張惟杰.糖復(fù)合多糖生化研究技術(shù)(第二版M.杭州:浙江大學(xué)出版社,1994.Box G E P。Hunter W G.Stati8tics for Experiment8:柚IntrDduction toDeBign,DataAn8lysis朋dModelBuildingM.NewYork:Wiley.1990.Optimi2,ation of Extraction of Lentinan by Response Surfa

6、ce Method0109ywEl zhenyuanl,zHoNc Yao-guan91-,LIuchang-jian礦(1.Co岫e o,d sc函撇n以,loz啊,髓肌g口i D促a幾踟咖睜,s們ghi201306;2.z婦e口廠d&如nce,sk吖。昭Ag廊曲umZ踟沁H妙,_sk,鄴喈,“咖llOl61Abstract:Response surfacemethodology(RSMwa8appliedtooptimize the hotwaterextr屺tions of lentinan.Extmction temper.atu陀,ex帆ction time and solid-“q

7、uid mtio we他selected鵝causal facto鴨.Basedonthesine faeto埔experiments,a tIlree.factor.tlIreeleveI exPeriments designweredeVeloped by BoxBenhnken centr8l comp08ite de8ign method.The yield of polysaccharide8wused舶the respon8ive valueB.The optimum hotwaterextraction conditions were船follow:extmction tempe

8、mtu咒i878.6、extmction time is 120minand tlIe 80lid-liquid is 1:58.3(g/m1.Under the optimum extraction technology condition,出e yieldof polysaccharides is 14.22%and the contend of polvsaccharides is 38.57%.KeywoHls:Lentin舳;ResponBe蛐dhce methodology;Extnction;0ptimiza“吲 響應(yīng)面優(yōu)化法對香菇多糖提取的工藝研究作者:魏楨元, 鐘耀廣, 劉長

9、江作者單位:魏楨元(上海海洋大學(xué)食品學(xué)院,上海,201306, 鐘耀廣(上海海洋大學(xué)食品學(xué)院,上海,201306;沈陽農(nóng)業(yè)大學(xué)食品學(xué)院,遼寧,沈陽,110161, 劉長江(沈陽農(nóng)業(yè)大學(xué)食品學(xué)院,遼寧,沈陽,110161刊名:遼寧農(nóng)業(yè)科學(xué)英文刊名:LIAONING AGRICULTURAL SCIENCES年,卷(期:2010(2參考文獻(7條1.Box G E P;Hunter W G Statistics for Experiments:an Introduction to Design,Data Analysis and Model Building 19902.張惟杰糖復(fù)合多糖生化研究技

10、術(shù) 19943.Furue H;Kitoh I Phase 111-study on Lentinan.Japanese 19814.Chihara G;Maeda YY;Hamuro J;Sasaki T,Fumiko F Inhibition of mouse sarcoma 180 by polysaccharides from Lentinus edodes 19695.ZHOU LD;ZHANG QH;ZHANG Y The shiitake mushroom-derived immune-stimulant lentinan protects against murine malaria blood-stage infection by evokin