亞鐵氰化鉀鐵氰化鉀放大碳量子點(diǎn)電化學(xué)發(fā)光對谷胱甘肽的測定表現(xiàn)出良好的靈敏性

1、文獻(xiàn)匯報(bào)研究背景：研究背景：谷胱甘肽（GSH）是重要的內(nèi)源性抗氧化劑，用于防御毒素。不正常的含量，減緩小孩的生長、頭發(fā)脫落、器官損傷，甚至糖尿病、心血管疾病等。因此谷胱甘肽（GSH）的檢測至關(guān)重要。碳量子點(diǎn)（C-dots）的優(yōu)點(diǎn)：電化學(xué)發(fā)光，生物兼容性。 碳量子點(diǎn)（C-dots）的缺點(diǎn)：狹窄的電勢窗口，減弱ECL信號。(11-pyrrolyl-1-ylundecyl) triethylammonium tetrafluoroborate (A2)作為陽離子表面活性劑通過靜電作用與C-dots結(jié)合在一起。亞鐵氰化鉀和鐵氰化鉀優(yōu)點(diǎn)：作為電子注入和空穴注入，能在電化學(xué)反應(yīng)中加快電子的轉(zhuǎn)移。亞鐵氰化鉀

2、和鐵氰化鉀在poly(C-dots/A2)能增強(qiáng)ECL信號。實(shí)驗(yàn)部分：制備C-dots2.673 g 丙氨酸溶解在30.0 ml 超純水再加入500 L 乙二胺轉(zhuǎn)入聚四氟乙烯高壓釜中 200加熱6 h冷卻至室溫，得到透明黑褐色液體在透析包（1000 MWCO）中透析24 h45下旋轉(zhuǎn)蒸發(fā)，得到粉末固體C-Dots固定在玻碳電極上：A2通過聲波降解法分散到二次蒸餾水中制得濃度為8 mMC-dotsC-dots/A2聲波降解10 minGCE/poly(C-dots/A2）取10 L，放在GCE上，室溫干燥 30 min。0-0.9 V；80圈；50 mV s-1。制備GCE/Poly(C-dot

3、s/A2)-Fe(CN)63-/4-GCE/poly(C-dots/A2）GCE/poly(C-dots/A)-Fe(CN)63-/4-浸入含有10 mM K3Fe(CN)6/K4Fe(CN)6 (1:1)0.1 M PBS(pH=7.0)。-0.4-0.7V；60圈；50mV s-1Figure 1. (A) CVs of GCE obtained in 0.1 M PBS (pH =7.0) containing 10 mM K4Fe(CN)6/K3Fe(CN)6 (a) and poly(C-dots/A2)-Fe(CN)63/4 in 0.1 M PBS (pH=7.0) (b) at

4、 a scan rate of 50 mV s1,respectively. (B) CVs of poly(C-dots/A2)-Fe(CN)63/4 in 0.1 M PBS (pH=7.0) at various scan rates. The scan rate from inner to outer is 50 (a), 100 (b), 200 (c), 300 (d), 400 (e), and 500 mV s1 (f). Inset: Plots of anodic and cathodic peak currents vs scan rates. (C) ECLpotent

5、ial curves of poly(C-dots/A2)-Fe(CN)63/4 in 0.05 M Tirs-HCl (pH=7.4) without (a) and with (b) 10 mM K2S2O8 at a scan rate of 100 mV s1. (D) ECL responses of poly(C-dots/A2)-Fe(CN)63/4obtained during a continuous potential scan between 1.75 and 0 V.電化學(xué)研究：電化學(xué)研究：Figure 2. (A) ECL-time curves of poly(C-

6、dots/A2)-Fe(CN)63/4 in the presence of (aj) 0, 0.1, 0.3, 0.5, 0.7, 1, 2, 4, 7, and 10 M GSH. The electrolyte is Tris-HCl (0.05 M, pH=7.4) with 10 mM K2S2O8 and 0.1 M KCl. (B) The corresponding ECL vs GSH concentration curves. Inset: Linear calibration plot for GSH detection. (C) The ECL response of

7、poly(C-dots/A2)-Fe(CN)63/4 in the presence of interfering substances.Figure 3. SEM (A,C,D) and TEM (B) images for (A) polyA2, (B) Cdots, (C) poly(C-dots/A2), and (D) poly(C-dots/A2) Fe(CN)63/4.Inset shows the photographs of C-dots/A2 solution under visible light (left) and under 365 nm UV light (rig

8、ht).Figure 4. (A) ECL (a) and PL (b) spectrum of the poly(C-dots/A2)-Fe(CN)63/4 system. (B) The effect of different coreactants on the ECL intensity of poly(C-dots/A2)-Fe(CN)63/4: (a) O2, (b) H2O2, (c) cysteine, and (d) K2S2O8. Inset: amplification of lines a, b, and c. (C) ECL response of poly(C-do

9、ts/A2), poly(C-dots/A2)-K4Fe(CN)6, poly(C-dots/A2)-K3Fe(CN)6, and poly(C-dots/A2)-Fe(CN)63/4 in Tris-HCl buffer solution (0.05 M, pH=7.4) with K2S2O8 (10 mM) and poly(C-dots/A2)-Fe(CN)63/4 in Tris-HCl buffer solution (0.05 M, pH=7.4) with K2S2O8 (10 mM) saturated by nitrogen.Figure 5. CVs of the mod

10、ified electrodes recorded in 0.1 M PBS (pH=7.0) for (A) GCE/poly(C-dots/A2) in the absence (a) and presence of O2 (b), (B) GCE/poly(C-dots/A2)-Fe(CN)63/4 in the absence (a) and presence of O2 (b), (C) under N2, GCE/poly(C-dots/A2)-Fe(CN)63/4 in the absence (a) and presence of 10 mM K2S2O8 (b), (D) under ambient atmosphere, GCE/poly(C-dots/A2) Fe(CN)63/4in the absenc