Introduction of Circulating Tumor Cells detectionCTC檢測的主要應(yīng)用

1、 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Introduction of Circulating Tumor Cells detection1 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. 循環(huán)腫瘤細(xì)胞（CTC，Circulating Tumor Cell）是存在于外周血中的各類腫瘤細(xì)胞的統(tǒng)稱，因

2、自發(fā)或診療操作從實體腫瘤病灶（原發(fā)灶、轉(zhuǎn)移灶）脫落，大部分CTC在進(jìn)入外周血后發(fā)生凋亡或被吞噬，少數(shù)能夠逃逸并錨著發(fā)展成為轉(zhuǎn)移灶，增加惡性腫瘤患者死亡風(fēng)險2Circulating Tumor Cells 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. 早期診斷 術(shù)后/化療后療效評估 復(fù)發(fā)風(fēng)險評估 預(yù)后評估 體內(nèi)耐藥性檢測 指導(dǎo)個體化治療CTC檢測的主要應(yīng)用3 2016 BD. BD, the BD Logo and all other tradema

3、rks are property of Becton, Dickinson and Company. CTC在PB中的比例太低CTC的半衰期短CTC的免疫分型指標(biāo)復(fù)雜多變4CTC檢測的難點 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. CTC在PB中的比例太低5CTC檢測的難點 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson an

4、d Company. CD45磁珠負(fù)選。 注意事項：降低非特異染色。（死細(xì)胞、FC blocking，管子封閉。）6分離/富集CTC常用方法 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. CellSearch System7First and only FDA approved test for enumerating CTCs 2016 BD. BD, the BD Logo and all other trademarks are proper

5、ty of Becton, Dickinson and Company. DAPI+CD45-EpCAM+CK8/18/19+8CellSearch檢測CTC使用指標(biāo) 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Flexible-easy to add more markersAccurate-based on phenotypeCompatible-similar procedure with TBNK and B27Economical-no

6、 need to buy another expensive machineFACS檢測CTC的優(yōu)點9 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. 10FACS檢測CTC的流程 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Workflow11Blood collectionPBMC isolatio

7、nLineage Cell DepletionStainingFACS detection and sortingIdentificationBD Vacutainer Venous Blood Collection BD Vacutainer CPT TubeBD IMag Human Lineage Cell Depletion SetBD PMG AntibodiesBD FACS/LSR analyzer and sorter 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickin

8、son and Company. 常用抗體12Antibodies特性特性CD45WBCEpCAM(CD326)上皮來源E-cadherin(CD324)CytokeratinsN-cadherin(CD325)間質(zhì)來源VimentinALDH1*干性指標(biāo)CD44CD24CD133*CD90 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. 測序/單細(xì)胞測序qPCR染色/單細(xì)胞染色CTC培養(yǎng)及功能驗證后續(xù)實驗13 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. 科研能力較強(qiáng)的醫(yī)院或與醫(yī)院密切合作的科研單位