GB/T 44965-2024飼料中β-阿樸-8′-胡蘿卜素酸乙酯的測定高效液相色譜法

ICS

65.120

CCS

B46

中華人民共和國國家標(biāo)準(zhǔn)

GB/T44965—2024

飼料中β?阿樸?8′?胡蘿卜素酸乙酯的

測定高效液相色譜法

Determinationofβ?apo?8′?carotenoicacidethylesterinfeeds—

Highperformanceliquidchromatography

2024-11-28發(fā)布2025-06-01實(shí)施

國家市場監(jiān)督管理總局發(fā)布

國家標(biāo)準(zhǔn)化管理委員會(huì)

GB/T44965—2024

目次

前言

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Ⅲ

1

范圍

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1

2

規(guī)范性引用文件

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3

術(shù)語和定義

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4

原理

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5

試劑或材料

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6

儀器設(shè)備

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7

樣品

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8

試驗(yàn)步驟

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8.1

試樣溶液的制備

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8.2

測定

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9

試驗(yàn)數(shù)據(jù)處理

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3

10

精密度

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4

附錄A（資料性）β?阿樸?8′?胡蘿卜素酸乙酯高效液相色譜圖

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5

Ⅰ

GB/T44965—2024

前言

本文件按照GB/T1.1－2020《標(biāo)準(zhǔn)化工作導(dǎo)則第1部分：標(biāo)準(zhǔn)化文件的結(jié)構(gòu)和起草規(guī)則》的規(guī)

定起草。

請(qǐng)注意本文件的某些內(nèi)容可能涉及專利。本文件的發(fā)布機(jī)構(gòu)不承擔(dān)識(shí)別專利的責(zé)任。

本文件由全國飼料工業(yè)標(biāo)準(zhǔn)化技術(shù)委員會(huì)（SAC/TC76）提出并歸口。

本文件起草單位：貴州省獸藥飼料檢測所、肇慶巨元生化有限公司、廣州智特奇生物科技股份有限

公司。

本文件主要起草人：趙貴、章厲劼、孫播東、王慶紅、蘇美丞、孫真崢、欒慶祥、黃鑫、朱基美、

鄧慶生、廖歡、王璇、金曉峰、謝麗麗、陳冰、肖峰、郭萍、周藝林、楊強(qiáng)。

Ⅲ

GB/T44965—2024

飼料中β?阿樸?8′?胡蘿卜素酸乙酯的

測定高效液相色譜法

1范圍

本文件描述了飼料中β?阿樸?8′?胡蘿卜素酸乙酯的高效液相色譜測定方法。

本文件適用于配合飼料、濃縮飼料、精料補(bǔ)充料和添加劑預(yù)混合飼料中β?阿樸?8′?胡蘿卜素酸乙酯

的測定。

本文件配合飼料、濃縮飼料、精料補(bǔ)充料中β?阿樸?8′?胡蘿卜素酸乙酯的檢出限為0.15mg/kg、

定量限為0.50mg/kg；添加劑預(yù)混合飼料中β?阿樸?8′?胡蘿卜素酸乙酯的檢出限為1.5mg/kg、定量限

為5.0mg/kg。

2規(guī)范性引用文件

下列文件中的內(nèi)容通過文中的規(guī)范性引用而構(gòu)成本文件必不可少的條款。其中，注日期的引用文

件，僅該日期對(duì)應(yīng)的版本適用于本文件；不注日期的引用文件，其最新版本（包括所有的修改單）適用

于本文件。

GB/T6682分析實(shí)驗(yàn)室用水規(guī)格和試驗(yàn)方法

GB/T20195動(dòng)物飼料試樣的制備

3術(shù)語和定義

本文件沒有需要界定的術(shù)語和定義。

4原理

試樣中β?阿樸?8′?胡蘿卜素酸乙酯經(jīng)水分散，用乙醇和二氯甲烷提取，固相萃取柱凈化（添加劑預(yù)

混合飼料除外），高效液相色譜儀測定，外標(biāo)法定量。

5試劑或材料

除非另有規(guī)定，僅使用分析純?cè)噭?/p>

5.1水：GB/T6682，一級(jí)。

5.2二氯甲烷。

5.3無水乙醇。

5.4乙腈：色譜純。

5.5甲醇：色譜純。

5.62,6?二叔丁基對(duì)甲酚（BHT）。

5.750%乙腈溶液：取100mL乙腈（5.4），用水稀釋至200mL，混勻。

5.8標(biāo)準(zhǔn)儲(chǔ)備溶液（200μg/mL）：減量法稱取β?阿樸?8′?胡蘿卜素酸