MCE IFN-γ 誘導蛋白 16 (IFI16) 的積累

MCEMasterofBioactiveMolecules客戶驗證誘導小鼠肺成纖維細胞纖維化TGF-β1(HY-P7117)刺激原代小鼠肺成纖維細胞，通過qRT-PCR和WB檢測PTPN13和NOX4表達水平，確定其與細胞纖維化密切相關(guān)[41]。ControlTGF-β1PTPN13NOX4expression10**expression8**86mRNA6mRNA44Relative2Relative200

ControlTGF-β1ControlTGF-β1CollagenI139kDaPTPN13310kDaα-SMA42kDaNOX455kDaGAPDH37kDaGAPDH37kDa構(gòu)建腎臟類器官從小鼠腎臟組織中提取細胞，利用微流體技術(shù)結(jié)合3D打印機制作類器官珠，輔以Noggin(HY-P7086)、R-spondin-1(HY-P7114)、FGF-4(HY-P7014)、FGF-basic(HY-P7066)、SB-431542(HY-10431)、Laduviglusib(HY-10182)培養(yǎng)，7天后，得到數(shù)千個大小、形狀和成分具有高度重復性的腎臟類器官[42]。OrganoidGeneratingKidneyOrganoidBeadsKidneyOrganoidsMatrigel+CellsDay1Day7QDs/Drugs7days24hrsOrganoidCulturingCulturingHighThroughputScreeningOil4°CKidneyOrganoidBeads200μm200μmCultureDish37°CIFN-γ誘導蛋白16(IFI16)的積累通過Westernblotting檢測IFN-γ(HY-P7025)處理人肺腺癌上皮(A549)細胞18小時后，蛋白16(IFI16)的積累，通過免疫染色檢測IFN-γ誘導16小時后IFI16的細胞定位[43]。HumanIFN-γ(ng/mL)0505001000IFI16—100(kDa)—70β-actin—40

NegativeControl IFN-γ(500ng/mL)DAPI/IFI1617www.MedChemEInhibitors ? ScreeningLibraries ? ProteinsMCE重組蛋白MCE重組蛋白產(chǎn)品種類豐富，涵蓋細胞因子、病毒蛋白、免疫檢查點蛋白、CAR-T蛋白、CD抗原、受體蛋白、生物素標記蛋白、GMP級蛋白、酶等多種分類，可用于人、病毒、小鼠、大鼠、猴、豬、狗、牛、羊、兔、貓、非洲爪蟾等多個物種不同研究領(lǐng)域的生物學研究，包括蛋白結(jié)構(gòu)研究、免疫檢測試劑、重組蛋白藥物、診斷試劑開發(fā)、抗體藥物靶點、CAR-T細胞治療靶點、Fc受體、流感病毒蛋白和細胞因子等熱門研究領(lǐng)域。MCE因高品質(zhì)的產(chǎn)品和優(yōu)質(zhì)的售前售后服務(wù)，成為全球數(shù)百萬計的科學家和技術(shù)人員值得信賴的實驗伙伴。產(chǎn)品種類豐富： 細胞因子和生長因子、免疫檢查點蛋白、CAR-T相關(guān)蛋白、CD抗原、受體蛋白、酶&調(diào)節(jié)子、補體系統(tǒng)蛋白、病毒類蛋白、生物素標記蛋白、熒光標記蛋白、GMP級蛋白,泛素相關(guān)蛋白等產(chǎn)品物種多樣性：人、病毒、小鼠、大鼠、猴、豬、狗、牛、羊、兔、貓、非洲爪蟾、細菌、真菌等低內(nèi)毒素水平： LAL法驗證，內(nèi)毒素含量低于絕大多數(shù)供應(yīng)標準高純度： SDS和HPLC檢測驗證高生物活性： 相關(guān)體內(nèi)/體外生物學實驗測定穩(wěn)定的一致性： 多批次檢測數(shù)據(jù)確認規(guī)格齊全： 滿足客戶的多種預分裝規(guī)格高純度ThepurityofhumanBMP-2isgreaterthan95%asanalyzedbySDSunderreducing(R)andnon-reducing(N)condition.kDa M R 120806040302010卓越的批間穩(wěn)定性TheED50ofMCEhumanVEGF165fromthreedifferentLotsaresimilar.1.0Lot1nm)0.8Lot20.6Lot3(4900.4OD0.2Net0.01010101010VEGF165,Human(ng/mL)

高活性HumanGM-CSFstimulatescellproliferationofTF-1cellswithanED50oflessthan0.5ng/mL.2.0(490nm)1.51.0NetOD0.50.010101010101010GM-CSF,Human(ng/mL)競品對比TheED50ofhumanVEGF165fromMCE’seachLotislowerthanofCompetitorP.1.0BrandPnm)0.8Lot20.6Lot3(4900.4OD0.2Net0.010-210-1101010VEGF165,Human(ng/mL)MCEMasterofBioactiveMoleculesPublicationsCitingUseofMCEProductsNature.2023Jun;618(7964):374-382.Nature.2023Jun;618(7966):862-870.Nature.2023Jun;618(7965):590-597.Nature.2023Jun;618(7963):180-187.Nature.2023May;617(7962):818-826Science.2023Jun9;380(6649):eabo2296.Science.2022Dec2;378(6623):eabo5503.Science.2022Nov18;378(6621):eabq7361.Science.2022Oct14;378(6616):eabq0132.Science.2022Jul8;377(6602):eabg9302Cell.2023Jun22;186(13):2823-2838.e20.Cell.2023May11;186(10):2144-2159.e22.Cell.2023Mar30;186(7):1352-1368.e18.Cell.2023Mar2;186(5):1026-1038.e20.Cell.2023Feb16;186(4):850-863.e16MCEGlobalPartnerswww.MedChemEInhibitors ? ScreeningLibraries ? 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