腦外傷后抑郁狀態(tài)模型的建立及效果評價

精品文檔-下載后可編輯腦外傷后抑郁狀態(tài)模型的建立及效果評價【摘要】目的探討建立腦外傷后抑郁狀態(tài)動物模型，并對此模型的建立和有效性做系統(tǒng)評價。方法本實驗在武漢大學(xué)醫(yī)學(xué)院實驗中心完成。選用48只雄性Wistar大鼠，隨機(jī)(隨機(jī)數(shù)字法)分為4組，每組12只。①假手術(shù)組：每籠6只飼養(yǎng)，正常飲食飲水；②模型組：以自由落體撞擊法制作大鼠左側(cè)額葉腦挫裂傷模型，每籠1只飼養(yǎng)，術(shù)后1周加以慢性應(yīng)激和孤養(yǎng)方法，造成腦外傷后抑郁狀態(tài)動物模型；③腦外傷組：以自由落體撞擊法制作大鼠左側(cè)額葉腦挫裂傷模型，每籠6只飼養(yǎng)，正常飲食飲水；④慢性不可預(yù)見性溫和應(yīng)激組：每籠1只飼養(yǎng)，采用慢性應(yīng)激方法結(jié)合孤養(yǎng)法造成大鼠慢性應(yīng)激抑郁模型。觀察各組大鼠蔗糖水試驗，自發(fā)改變，用高效液相色譜熒光檢測法測定各組大鼠腦內(nèi)海馬、前額葉、下丘腦和紋狀體不同部位的多巴胺、5-羥色胺、去甲腎上腺素單胺類神經(jīng)遞質(zhì)含量。采用SPSS13.0統(tǒng)計軟件分析結(jié)果，資料采用單因素方差分析或成組t檢驗，以P<0.05為差異具有統(tǒng)計學(xué)意義。結(jié)果造模后即予行為學(xué)測試，模型組大鼠蔗糖水飲用量逐漸減少，反映模型組大鼠興趣和缺乏；曠野試驗中模型組大鼠水平及垂直得分減少，反映模型組大鼠活動性減少，興趣缺乏；模型組大鼠游到終點的時間延長，錯誤次數(shù)增加，反映模型組大鼠的學(xué)習(xí)和記憶能力下降；模型組大鼠海馬、前額葉、下丘腦，和紋狀體多巴胺、5-羥色胺、去甲腎上腺素含量下降。結(jié)論在左側(cè)額葉腦挫裂傷模型的基礎(chǔ)上給予孤養(yǎng)和慢性不可預(yù)見的溫和性應(yīng)激，能建立較理想的腦外傷后抑郁狀態(tài)動物模型。

【關(guān)鍵詞】腦外傷；抑郁；動物模型;單胺類神經(jīng)遞質(zhì);形態(tài)學(xué)

EstablishmentandevaluationofmodelofdepressionaftertraumaticbraininjuryCHUSheng-hua,CHENEr-tao,FENGDong-fu,MAYan-bin,ZHUZhi-an.DepartmetofNeurosurgery,NO.省略

【Abstract】ObjectiveTodiscussabouttheestablishmentofmentaldepressionmodelbytraumaticbraininjuryinrats，andtoevaluatethevalidityofthismodel.MethodsThestudywascarriedoutintheexperimentcenterofWuhanUniversity.Atotalof48maleWistarratswererandomized（randomnumber）intofourgroups(n=12,each):(1)inshamoperationgroup,sixratswerefednormallyinonecage;(2)inmodelgroup,themodeloftheleftfrontallobecontusionwasmadeupbyhittingwithfree-fallmethodontheleftcortexoftherat,andsubsequentlytheeachofmodelratswereseparatelyfedinacageandputtheminalonelyenvironmentwithchronicstressoneweekaftertraumaticbraininjuryinordertoinducethemintomodelsofmentaldepression;(3)inbraintraumagroup,thetraumamodeloftheleftfrontallobecontusionwassetupbythesameprocedureasinmodelgroup,andsixratswerefedtogetherinonecage;(4)inCUMSgroup,eachratwasfednormallyinonecageinalonelyenvironmentafterbraintraumamadeandchronicmildchaoticstressunpredictabletoratswasgiventoinducementaldepression.Theconsumptionofsucrosewaterandthechangeofanimalbehaviorwereobservedandthehighperformanceliquidphaseelectrochemicalwithfluorescencedetectorwasusedtodetectthebiogenicmonoamineneurotransmittercontent(dopamine,serotoninandnorepinephrine)inthehippocampus,prefrontalcortex,hypothalamusandstriatumareaineachgroup.ExperimentaldatawereprocessedwithsinglefactoranalysisorttestofvariancebyStatisticalProgramforSocialSciencesVersion13.0(SPSS13.0)software.P<0.05wasconsideredasstatisticallysignificantdifference.ResultsAftermodeling,theratsoffourgroupswereexaminedbybehavioraltests.Itwasfoundthattheamountofsugar-waterconsumptionbytheratsinthemodelgroupandCUMPgroupdecreased,implyingtheabsenceofinterestineatingandanhedoniaoftheratsinthesegroups.Inopen-fieldtest,theratsinthemodelgroup,CUMPgroupandbraintraumagroupshowedreductioninsquarecrossingandrearing,implyingtheunder-activityandabsenceofinterestinactivity.Inthewatermazetest,itwasfoundthattheratsinthemodelgroup,CUMPgroupandbraintraumagroupneededmuchtimetoreachtheend-pointandmademanyerrors,implyingtheloweringabilitytolearnandmemorize.Levelsofdopamine,serotoninandnorepinephrineinthehippocampus,prefrontalcortex,hypothalamusandstriatumareadecreasedintheseratswithtraumatizedbraininthreegroupsespeciallyinmodelgroupandCUMPgroup.ConclusionsItisavalidmethodforestablishingthementaldepressionmodelinrat