RT-AM-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemERT-AMCat.No.:HY-108715ACASNo.:2280796-94-1分?式:C??H??N?O??分?量:555.49作?靶點(diǎn):Others作?通路:Others儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:25mg/mL(45.01mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM1.8002mL9.0011mL18.0021mL5mM0.3600mL1.8002mL3.6004mL10mM0.1800mL0.9001mL1.8002mL請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲(chǔ)存時(shí)，請?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請?jiān)?個(gè)?內(nèi)使?。BIOLOGICALACTIVITY?物活性RT-AMrealthiol前體藥物。RealThiol?種于可逆反應(yīng)的熒光探針，可以定量監(jiān)測活細(xì)胞中?胱?肽的實(shí)時(shí)動(dòng)態(tài)變化。體外研究ToenhancethecellpermeabilityofRealThiol,thecarboxylicacidgroupsisconvertedtoacetoxymethyl(AM)esters,whicharereadilyhydrolysedbyesterasestoregenerateRealThiolinsidecells.Forcellstreatedwith1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEtheRT-AM,real-timeratiometricimagesoftherapidchangesofintracellularGSHconcentrationsinsinglecellscanbegeneratedbydividingthefluorescenceintensityvaluesforthe405?nmchannelbythe488?nmchannelateachcorrespondingpixel[1].PROTOCOLCellAssay[1]TheHeLacelllineisgrowninDMEMmediasupplementedwith10%FBSand1%1003PenStrep.Cellsareculturedunderacontrolledatmosphere(37°C,5%CO2).Glassbottomdishesareusedforcellcultureduetoconfocalscanningrequirements.CellsaretreatedwithRT-AM(1?μMwith1%DMSOinDMEM)for10-15?minbeforeimaging.Confocalimagesareacquiredwith405?nmlaser/418–495?nmfilterand488?nmlaser/499–615?nmfilter.Allthemicroscopesettingsarekeptconsistentineach[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?EnvironSciTechnol.2022Aug10.?EnvironPollut.2021Oct7;118296.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].JiangX,etal.Quantitativereal-timeimagingofglutathione.NatCommun.2017Jul13;8:16087.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Te