Propidium-Iodide-PI-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEPropidiumIodideCat.No.:HY-D0815CASNo.:25535-16-4Synonyms:PI分?式:C??H??I?N?分?量:668.39作?靶點(diǎn):Others作?通路:Others儲(chǔ)存?式:4°C,sealedstorage,awayfrommoistureandlight*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoistureandlight)溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:100mg/mL(149.61mM;Needultrasonic)H2O:3.57mg/mL(5.34mM;ultrasonicandwarmingandheatto60°C)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM1.4961mL7.4807mL14.9613mL5mM0.2992mL1.4961mL2.9923mL10mM0.1496mL0.7481mL1.4961mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；?旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存?式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoistureandlight)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)?內(nèi)使?。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案。以下溶解?案都請(qǐng)先按照InVitro?式配制澄的儲(chǔ)備液，再依次添加助溶劑：(為保證實(shí)驗(yàn)結(jié)果的可靠性，澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的?式助溶)1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%saline1/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemESolubility:≥2.5mg/mL(3.74mM);Clearsolution2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(3.74mM);ClearsolutionBIOLOGICALACTIVITY?物活性PropidiumIodide可?于細(xì)胞染?的紅?熒光染料。體外研究PropidiumIodideisacell-membraneimpermeabledyewithcharacteristicexcitationmaximumat535nmandemissionmaximumat617nmwhichintercalateswithnucleicacidswithastoichiometryofonedyeper4-5basepairswithlittlesequencepreference.PropidiumIodidehasevidencedofhavingnotoxiceffectsonneurons,beingtoday’smostcommonmarkerformembraneintegrityandcellviabilitywhenappliedpriortofixation(pre-fixationPropidiumIodidestainingmethod).Thepre-fixationstaininghasbeenwidelyusedforquantitativeassessmentsofneuronalcelldeclineinmodelsofacuteneurodegeneration,visualizedasintenselylabeledPI+-pycnoticnucleiofdegeneratingneurons[1].PropidiumIodidecannotcrossthemembraneoflivecells,makingitusefultomeasurethepercentageofapoptoticcellsbyflow-cytometricanalysis.Theflowcytometricdatashowsanexcellentcorrelationwiththeresultsobtainedwithbothelectrophoreticandcolorimetricmethods.Thisnewrapid,simpleandreproduciblemethodprovesusefulforassessingapoptosisofspecificcellpopulationsinheterogeneoustissuessuchasbonemarrow,thymusandlymphnodes[2].PROTOCOLCellAssay[2]Flowcytometricanalysis:Propidiumiodideispreparedinin0.1%sodiumcitrateplus0.1%TritonX-100(50μg/mL).The200×gcentrifugedcellpelletisgentlyresuspendedin1.5mLhypotonlcfluorochromesolution(Propidiumiodide50μg/mL),in12×75polypropylenetubes.Thetubesareplacedat4°Cinthedarkovernightbeforetheflowcytometricanalysis.ThepropidiumIodidefluorescenceofindividualnucleiismeasuredusingaFACScanflowcytometer.Thenucleitraversesthelightbeamofa488nmArgonlaser.A560nmdichrolcnurror(DM570)anda600nmbandpassfilter(bandwidth35nm)areusedforcollectingtheredfluorescenceduetopropidiumIodidestainingofDNAandthedataareregisteredonalogarithmicscale[2].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.戶使?本產(chǎn)品發(fā)表的科研?獻(xiàn)?BioactMater.2022Aug11;21:20-31.?Small.2022Jul;18(30):e2202002.?JAdvRes.22December2021.?RedoxBiol.2022Feb;49:102207.?CellRep.2022Aug23;40(8):111240.Seemorecustomervalidationsonwww.MedChemE2/3MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEREFERENCES[1].HezelM,etal.Propidiumiodidestaining:anewapplicationinfluorescencemicroscopyforanalysisofcytoarchitectureinadultanddevelopingrodentbrain.Micron.2012Oct;43(10):1031-8.[2].Arapidandsimplemethodformeasuringthymocyteapoptosisbypropidiumiodidestainingandflowcytometry.JImmunolMethods.1991Jun3;139(2):271-9.McePdfHeigh