Affymerix基因芯片產(chǎn)品

Affymetrix基因芯片產(chǎn)品Upto~7,000,000features

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chip5-11μmRNAtargetThousandsofcopiesofaspecificoligonucleotideprobeineachfeatureRawdataimagewith1-6.9

milliondifferentcomplementaryprobes

Oligonucleotideprobe*****1.28cmHybridizedprobecell*5”5”芯片產(chǎn)品分類表達(dá)譜芯片-mRNA3’IVTWTMicroRNA芯片lncRNA芯片SNP/CNV芯片甲基化&轉(zhuǎn)錄因子芯片表達(dá)譜芯片3’IVTHumanU133Plus2.0MouseU4302.0RatU2302.0PrimeView&AlmacXcelWTHuman/Rat/MouseGene1.0STHuman/Rat/MouseExon1.0STHTA2.0表達(dá)譜芯片探針設(shè)計Exon1.0ST基因組DNA初始轉(zhuǎn)錄本RNA可變剪切轉(zhuǎn)錄本Gene1.0ST3’端表達(dá)譜表達(dá)譜芯片實驗步驟樣本RNA抽提&質(zhì)檢cDNA合成及片段化生物素標(biāo)記芯片雜交-洗脫-掃描芯片數(shù)據(jù)標(biāo)準(zhǔn)化Gene系列芯片TranscriptcoverageofthearrayHumanGene1.0STArrayHumanGene2.0STArrayTotalRefSeqtranscripts

covered36,07940,716NM?RefSeqcodingtranscript,

well-establishedannotation32,02030,654NR?RefSeqnon-codingtranscript,

wellestablishedannotation2,9675,638XM?RefSeqcodingtranscript,

provisionalannotation579996XR?RefSeqnon-codingtranscript,provisionalannotation5133,428lincRNAtranscripts46611,086RefSeq(Entrez)genecount21,01424,838EXON系列芯片DesignStatisticsSummaryProbeSets1.4millionExonClusters>1millionSupportedbyPutativeFull-LengthmRNA289,961probesetsSupportedbyEnsemblTranscripts306,583probesetsSupportedbyEST665,175probesetsSupportedbyMouseorRatmRNA220,262probesetsSupportedbyGenePrediction883,105probesetsProbeSelectionRegionAlongtheentirelengthofthetranscriptsProbes/ProbeSelectionRegion4BackgroundSubtractionStrategyMedianintensityofupto1,000backgroundprobeswiththesameGCcontentTotalFeaturesPerArray>5,500,000InterrogatedStrandSense2HTA2ArraycontentProteincodingcontentNon-proteincodingcontentGenes(transcriptclusters)44,69922,829Transcripts245,34940,914Exons560,472109,930Exonclusters296,05882,444Splicejunctions(probesets)339,1460表達(dá)譜芯片應(yīng)用U133——經(jīng)典，相對舊，無價格優(yōu)勢，不做推薦Gene系列——基本，僅滿足一般表達(dá)量檢測需求Exon系列——全面，精確表達(dá)量檢測HTA2——最佳，最全&最精確表達(dá)量EG1.0——最佳，唯一能夠獲得轉(zhuǎn)錄本表達(dá)量MicroRNA芯片MicroRNA4.0microRNAPre-microRNAsnoRNA&scaRNAmicroRNA芯片實實驗步步驟樣本RNA抽提&質(zhì)檢Poly(A)加尾&FlashTagBiotinHSR連接生物素素標(biāo)記記芯片雜雜交-洗脫-掃描芯片數(shù)數(shù)據(jù)標(biāo)標(biāo)準(zhǔn)化化microRNA4.0特點(diǎn)100%覆蓋miRBaseV20包括所所有數(shù)數(shù)據(jù)庫庫記錄錄的物物種人2578小鼠1908大鼠728重復(fù)性性>0.95技術(shù)重重復(fù)生物重重復(fù)lncRNA芯片Human/Mouse/RatGene2.0HTA2.0(Human)EG1.0-Human/MouseLNCRNA芯片實實驗流流程樣本RNA抽提&質(zhì)檢cDNA合成及片段段化生物素素標(biāo)記記芯片雜雜交-洗脫-掃描芯片數(shù)數(shù)據(jù)標(biāo)標(biāo)準(zhǔn)化化SNP/CNV芯片SNP6.0CytoScan750KCytoScanHDOncoScan訂制芯芯片Cyto芯片適適用樣樣本細(xì)胞組織血液唾液骨CytoHD高覆蓋率270萬CNV探針75萬SNP探針高性能特異性靈敏度高分辨率~1marker/1-3KB~1marker/5KB純合雜合父本母本子代Cyto系列芯芯片價價值雜合性缺失單親二倍體三倍體樣本異質(zhì)性家系分析甲基化化芯片片HumanPromoter1.0RDNA甲基化CpG島在CpG甲基化結(jié)合蛋白和DNA甲基化轉(zhuǎn)移酶的作用下，使CpG二核苷酸5’端的胞嘧啶轉(zhuǎn)變成為5’甲基胞嘧啶。通常是基因的啟動子區(qū)域，5’端非翻譯區(qū)和第一個外顯子區(qū)，CpG序列密度非常高，超過均值5倍以上，為鳥嘌呤和胞嘧啶的富集區(qū)。研究甲甲基化化意義義腫瘤DNA甲基化過低CpG島高甲基化發(fā)育出生后發(fā)育衰老整體甲基化水平樣品交聯(lián)染色質(zhì)片段化CHIP洗滌純化PCR擴(kuò)增DNA片段化標(biāo)記雜交洗脫掃描Pr