ML188-DataSheet-生命科學(xué)試劑-MedChemExpress

Hotline:400-820-3792Inhibitors?Agonists?ScreeningLibrarieswww.MedChemEML188Cat.No.:HY-136259CASNo.:1417700-13-0分?式:C??H??N?O?分?量:433.54作?靶點(diǎn):SARS-CoV;VirusProtease作?通路:Anti-infection儲(chǔ)存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數(shù)據(jù)體外實(shí)驗(yàn)DMSO:250mg/mL(576.65mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲(chǔ)備液1mM2.3066mL11.5330mL23.0659mL5mM0.4613mL2.3066mL4.6132mL10mM0.2307mL1.1533mL2.3066mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存?式和期限。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥?式選擇適當(dāng)?shù)娜芙?案，配制前請(qǐng)先配制澄的儲(chǔ)備液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)實(shí)驗(yàn)的?作液，建議您現(xiàn)?現(xiàn)配，當(dāng)天使?；澄的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；以下溶劑前的百分?指該溶劑在您配制終溶液中的體積占?)：1.請(qǐng)依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥6.25mg/mL(14.42mM);Clearsolution2.請(qǐng)依序添加每種溶劑：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥6.25mg/mL(14.42mM);Clearsolution3.請(qǐng)依序添加每種溶劑：10%DMSO>>90%cornoilSolubility:≥6.25mg/mL(14.42mM);Clearsolution1/2MasterofSmallMolecules—您?邊的抑制劑?師www.MedChemEBIOLOGICALACTIVITY?物活性ML188，?種探針，也?種選擇性?共價(jià)SARS-CoV3CLpro抑制劑，IC50為1.5μM。具有抗病毒活性。體外研究ML188(0-30μM;48hours)effectivelyinhibitSARS-CoVreplicationincellculture[1].ProbeML188isamodestmolecularweightSARS-CoV3CLproinhibitorwithdemonstratedantiviralactivityandanon-covalentmechanismofaction.ML188yieldsanantiviralEC50valuerangingfrom12.9to13.4μMinmock-infectedandSARS-CoVinfectedcells[1].CellViabilityAssay[1]CellLine:VeroE6cells(mock-infectedorinfectedwithSARS-CoV)Concentration:3,4,5,8,10,15,20,30μMIncubationTime:48hourResult:Againstmock-infectedandSARS-CoVinfectedcells.?bioRxiv.2020Jul.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].JacobsJ,etal.Discovery,synthesis,andstructure-basedoptimizationofaseriesofN-(tert-butyl)-2-(N-arylamido)-2-(pyridin-3-yl)acetamides(ML188)aspotentnoncovalentsmallmoleculeinhibitorsofthesevereacuterespiratorysyndromecoronavirus(SARS-CoV)3CLprotease.JMedChem.2013Jan24;56(2):534-46.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonl