JC-1-CBIC2-DataSheet-生命科學(xué)試劑-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEJC-1Cat. No.: HY-15534CAS No.: 3520-43-2Synonyms: CBIC2分式: CHClIN分量: 652.23作靶點(diǎn): Others作通路: Others儲(chǔ)存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight)溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 15 mg/mL (23.00 mM)H2O

2、: 0.1 mg/mL (insoluble)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 1.5332 mL 7.6660 mL 15.3320 mL5 mM 0.3066 mL 1.5332 mL 3.0664 mL10 mM 0.1533 mL 0.7666 mL 1.5332 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。BIOLOGICAL ACTIVITY物活性 JC-1熒光親脂性羰花 染料，于測(cè)量線粒

3、體膜電位。體外研究JC-1 (2.5M) exposed to murine L1210 lymphoblasts, can be detected the presence of both cytoplasmic JC-1 monomer and mitochondrial J-aggregates in these cells. JC-1 fluorescence is usually excited by the 488nm laser wavelength common in flow cytometers 1. Fluorescent labeling of mitochondria

4、 with either JC-1 (11/2 Master of Small Molecules 您邊的抑制劑師www.MedChemEg/mL, 15 min), reveals that are distributed irregularly, resulting in regions of high and low mitochondrialcontent within astrocytes 2. JC-1 has been shown to interact with -synuclein at the acidic C-terminal regionwith a Kd of 2.6

5、 M. JC-1 itself does not accelerate the protein aggregation of -synuclein in the absence ofiron, insted, it decelerates the aggregation process by extending the lag phase approx 3. JC-1 is avidlyaccumulated in sensitive K562 cells where it displays both a green cytoplasmic and red mitochondrialfluor

6、escence. JC-1 is poorly accumulated in resistant K562 cells, which displays only a slight greenfluorescence 4.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Small. 2019 Jul 28:e1902642. Mol Cancer. 2019 Apr 10;18(1):85. Life Sci. 2018 Jun 15;203:291-304. Chem Res Toxicol. 2018 Nov 19;31(11):1164-1171. New J Chem. 18 Oct 2017.See more

7、 customer validations on HYPERLINK / www.MedChemEREFERENCES1. A Perelman, et al. JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry. Cell Death Dis. 2012Nov 22;3:e430.2. Vera C. Keil, et al. Ratiometric high-resolution imaging of JC-1 fluorescence reveals

8、the subcellular heterogeneity of astrocyticmitochondria. Pflgers Archiv - European Journal of Physiology. 2011,462(5): 693-708.3. Jung-Ho LEE, In-Hwan LEE, Young-Jun CHOE, et al. Real-time analysis of amyloid fibril formation of -synuclein using a fibrillation-state-specific fluorescent probe of JC-

9、1. Biochem. J. 2009, 418:311-323.4. Salvioli S, et al. JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells:implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 1997 Jul 7;411(1):77-82.McePdfHeightCaution: Product has not been fully validated for medica