鹽酸甲基芐肼作用機(jī)制 - Medchemexpress - MCE中國(guó)

1、Product Data SheetProcarbazine HydrochlorideCat. No.: HY-13733CAS No.: 366-70-1分式: CHClNO分量: 257.76作靶點(diǎn): DNA Alkylator/Crosslinker作通路: Cell Cycle/DNA Damage儲(chǔ)存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 16.67 mg/mL (64.67 mM; Need ultrasonic)SolventMass1 mg 5 m

2、g 10 mgConcentration制備儲(chǔ)備液1 mM 3.8796 mL 19.3979 mL 38.7958 mL5 mM 0.7759 mL 3.8796 mL 7.7592 mL10 mM 0.3880 mL 1.9398 mL 3.8796 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。儲(chǔ)備液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?6 個(gè)內(nèi)使，-20C 儲(chǔ)存時(shí)，請(qǐng)?jiān)?1 個(gè)內(nèi)使。體內(nèi)實(shí)驗(yàn)請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍?。以下溶解案都?qǐng)先按照 In

3、 Vitro 式配制澄清的儲(chǔ)備液，再依次添加助溶劑：為保證實(shí)驗(yàn)結(jié)果的可靠性，澄 的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使； 以下溶劑前顯的百分 指該溶劑在您配制終溶液中的體積占；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的式助溶1. 請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 1.67 mg/mL (6.48 mM); Clear solution此案可獲得 1.67 mg/mL (6.48 mM，飽和度未知) 的澄清溶液。以 1 mL 作液為例，取 100 L

4、16.699999 mg/mL 的澄清DMSO 儲(chǔ)備液加到 400 L PEG300 中，混合均勻；向上述體系中加 50 L Tween-80，混合均勻；然后繼續(xù)加 450 L 理鹽定容 1 mL。2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 1.67 mg/mL (6.48 mM); Clear solution此案可獲得 1.67 mg/mL (6.48 mM，飽和度未知) 的澄清溶液。Page 1 of 2 www.MedChemE以 1 mL 作液為例，取 100 L 16.699999 mg/mL 的澄，混合

5、均勻。DMSO 儲(chǔ)備液加到 900 L 20% 的 SBE-CD 理鹽溶液中3. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 1.67 mg/mL (6.48 mM); Clear solution此案可獲得 1.67 mg/mL (6.48 mM，飽和度未知) 的澄 溶液，此案不適于實(shí)驗(yàn)周 期在半個(gè)以上的實(shí)驗(yàn)。以 1 mL 作液為例，取 100 L 16.699999 mg/mL 的澄 DMSO 儲(chǔ)備液加到 900 L 油中，混合均勻。BIOLOGICAL ACTIVITY物活性 Procarbazine Hydrochloride種烷化劑 (al

6、kylator)，具有抗腫瘤的作。體外研究 Procarbazine Hydrochloride is an anticancer agent. Procarbazine is not cytotoxic to the LI 210 cells which lackcytochrome P-450 or monoamine oxidase activity1.體內(nèi)研究 Procarbazine Hydrochloride (50 mg/kg, i.p.) causes micronuclei in hematopoietic cells, but does not increase the l

7、acZmutant frequency (MF) in bone marrow of mice, similar to that in liver, testis, spleen, kidney, and lung. ProcarbazineHydrochloride (50 mg/kg, i.p.) has positive effect on lung, bone marrow, and spleen for carcinogenesis2.Procarbazine (450 mg/kg) significantly decreases testicular and epididymal

8、weight and drastically reduces haploidcells and spermatogenic arrest in hamster3.PROTOCOLCell Assay 1 Following Procarbazine or metabolite treatment, cells are diluted to 50.000/mL in 25-cm2 culture flasks (10 mL).Every 24 h, a 0.5-mL aliquot is removed, diluted 20-fold in Hematall isotonic diluent,

9、 and the cell number determinedwith a Coulter Model F electronic cell counter. Counts greater than 10,000/0.5 mL are corrected for coincidence. Cellsare diluted in fresh culture media when cell density exceeded 1 106/mL. Cultures are maintained until theaggregate cell number approached 100 106/mL an

10、d doubling time has returned to 12 h. Cell survival is determinedusing Equation A, where TD(doubling time for cells of interest) is 12 h1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Mice2Administration 2 Male MutaTM Mouse animals (7-8 weeks o

11、ld) are used after 2 weeks of acclimation. In the first experiment, 18 miceare injected intraperitoneally (i.p.) with 50 mg/kg Procarbazine hydrochloride in 10 mL saline/kg and eight mice areinjected with 10 mL saline/kg as the vehicle control. Six treated mice are killed 7, 14, and 28 days after tr

12、eatment,and four control mice are killed 7 and 28 days after the treatment. Killing is by cervical dislocation2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶(hù)使本產(chǎn)品發(fā)表的科研獻(xiàn) J Mol Med (Berl). 2019 Aug;97(8):1183-1193. Mol Imaging Biol. 2019 Apr 15.See mor

13、e customer validations on HYPERLINK www.MedChemE www.MedChemEPage 2 of 3 www.MedChemEREFERENCES1. Erikson JM, et al. Cytotoxicity and DNA damage caused by the azoxy metabolites of procarbazine in L1210 tumor cells. Cancer Res. 1989 Jan 1;49(1):127-33.2. Suzuki T, et al. Procarbazine genotoxicity in the MutaMouse; strong clastogenicity and organ-specific induction of lacZ mutations. Mutat Res. 1999 Aug18;444(2):269-81.3. Weissenberg R, et al. Procarbazine effects on spermatogenesis in golden hamster: a flow cytometric evaluation. Arch Androl. 2002 Mar-Apr;48(2):91-100.McePdfHeightC